Histone H4 (acetyl K5 + K8 + K12 + K16) Recombinant Rabbit mAb (S-902-37)

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0878

规格

25μl100μl1ml

数量

产品介绍

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产品规格

宿主来源

Rabbit
抗原名称

Histone H4 (acetyl K5 + K8 + K12 + K16)
免疫原

Synthetic Peptide
细胞定位

Nucleus
Accession

P62805
克隆号

S-902-37
抗体类型

Recombinant mAb
抗体同种型

IgG
翻译后修饰类型

乙酰化
反应种属 ?

Hu, Ms, Rt
预测反应种属
(反应种属缩写表)

Fu, Dr, Pl, Ys
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

ChIP

IHC-P ?

ICC ?

WB
稀释度

应用稀释度推荐种属

Dot Blot 1:1000
WB 1:1000 Hu, Ms
IHC-P 1:200 Hu, Ms, Rt
ICC 1:500 Hu
ChIP 1:20~1:50 Hu

应用	稀释度	推荐种属
Dot Blot	1:1000
WB	1:1000	Hu, Ms
IHC-P	1:200	Hu, Ms, Rt
ICC	1:500	Hu
ChIP	1:20~1:50	Hu

背景介绍

Acetylated histones are a key component of the epigenetic regulation of gene expression in eukaryotic cells. Histone acetylation involves the addition of an acetyl group to the lysine residues on the histone proteins that make up the nucleosome, which is the basic unit of chromatin. This process is generally associated with transcriptional activation, as it loosens the interaction between the DNA and the histones, allowing the transcription machinery to access the DNA and read the genetic information. Abnormalities in histone acetylation, such as global hypoacetylation or hyperacetylation, can lead to dysregulation of gene expression and have been implicated in various diseases, including cancer. Understanding the role of acetylated histones in gene regulation is crucial for developing therapeutic strategies for these diseases.

免疫印迹
- WB result of Histone H4 (acetyl K5 + K8 + K12 + K16) Recombinant Rabbit mAb
  Primary antibody: Histone H4 (acetyl K5 + K8 + K12 + K16) Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: untreated HeLa whole cell lysate 20 µg
  Lane 2: HeLa treated with 500 ng/ml TSA for 4 hours whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 11 kDa
  Observed MW: 13 kDa
- WB result of Histone H4 (acetyl K5 + K8 + K12 + K16) Recombinant Rabbit mAb
  Primary antibody: Histone H4 (acetyl K5 + K8 + K12 + K16) Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: untreated NIH/3T3 whole cell lysate 20 µg
  Lane 2: NIH/3T3 treated with 500 ng/ml TSA for 4 hours whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 11 kDa
  Observed MW: 13 kDa
免疫沉淀
斑点杂交
- Dot blot result of Histone H4 (acetyl K5 + K8 + K12 + K16) Recombinant Rabbit mAb
  Lane 1: Histone H4 (acetyl K5 + K8 + K12 + K16) peptide
  Lane 2: Histone H4 (acetyl K5) peptide
  Lane 3: Histone H4 (acetyl K8) peptide
  Lane 4: Histone H4 (acetyl K12) peptide
  Lane 5: Histone H4 (acetyl K16) peptide
  Lane 6: Histone H4 unmodified peptide
  Primary antibody: Histone H4 (acetyl K5 + K8 + K12 + K16) Recombinant Rabbit mAb at 1/1000 dilution
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
免疫组化
- IHC shows positive staining in paraffin-embedded human colon. Anti-Histone H4 (acetyl K5 + K8 + K12 + K16) antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human kidney. Anti-Histone H4 (acetyl K5 + K8 + K12 + K16) antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human breast cancer. Anti-Histone H4 (acetyl K5 + K8 + K12 + K16) antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-Histone H4 (acetyl K5 + K8 + K12 + K16) antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse stomach. Anti-Histone H4 (acetyl K5 + K8 + K12 + K16) antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat stomach. Anti-Histone H4 (acetyl K5 + K8 + K12 + K16) antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
- ICC analysis of HeLa cells treated with TSA (500ng/ml,4h) (top panel) and HeLa cells untreated with TSA (500ng/ml,4h) (below panel). Anti- Histone H4 (acetyl K5 + K8 + K12 + K16) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ChIP
- Chromatin immunoprecipitation (ChIP) was
  performed on HeLa+TSA (500ng/ml,4h) (+) cells
  cross - linked with 1% formaldehyde for 10 min,
  then chromatin was fragmented by sonication.
  Parallel reactions used Histone H4 (acetyl K5 +
  K8 + K12 + K16) Recombinant Rabbit mAb (S-902-37)
  and Rabbit mAb IgG Isotype Control (SDT-R173)
  at 1:50 for immunoprecipitation.
  Post - immunoprecipitation, both samples
  were washed, eluted, and cross - links
  reversed. Purified DNA was analyzed by qPCR.
  qPCR (%input: immunoprecipitated DNA/input DNA)
  showed the enrichment of RPL30, GAPDH, MYOD1,
  AFM, SAT-α and SAT-2 in Histone H4 (acetyl K5 + K8 +
  K12 + K16) Recombinant Rabbit mAb (S-902-37)
  -immunoprecipitated sample.