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Rabbit抗原名称
IL-2分子别名
Interleukin-2, T-cell growth factor (TCGF)免疫原
Recombinant Protein细胞定位
SecretedAccession
P60568克隆号
S-146-223抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
WB
稀释度
应用 稀释度 WB 1:1000
Interleukin 2 (IL-2) is a cytokine that produced predominantly by Th cells, stimulates the growth and differentiation of B cells, T cells, NK cells, and monocyte/macrophages. IL-2 signals through a receptor complex consisting of the IL-2Rα subunit, IL-2Rβ subunit, and γc, which activates the JAK/STAT pathway, especially STAT3 and 5, leading to gene transcription.
免疫印迹
WB result of IL-2 Recombinant Rabbit mAb
Primary antibody: IL-2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated Jurkat whole cell lysate 20 µg
Lane 2: Jurkat treated with 40 nM Phorbol-12-myristate-13-acetate, 2 µM A23187, and 300 ng/ml Brefeldin A (45 min delay) overnight whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 17 kDa
Observed MW: 15 kDa
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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