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ROCK1+ROCK2 Recombinant Rabbit mAb (S-1305-26)

Rho-associated protein kinase 1,Renal carcinoma antigen NY-REN-35,Rho-associated coiled-coil-containing protein kinase 1,Rho-associated coiled-coil-containing protein kinase I (ROCK-I),p160 ROCK-1 (p160ROCK),Rho-associated protein kinase 2,Rho kinase 2,Rho-associated,coiled-coil-containing protein kinase 2,coiled-coil-containing protein kinase II (ROCK-II),p164 ROCK-2

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0868

规格

25μl100μl1ml

数量

产品介绍

产品介绍 FAQs 评论(0)

产品规格

宿主来源

Rabbit
抗原名称

ROCK1+ROCK2
分子别名

Rho-associated protein kinase 1, Renal carcinoma antigen NY-REN-35, Rho-associated coiled-coil-containing protein kinase 1, Rho-associated coiled-coil-containing protein kinase I (ROCK-I), p160 ROCK-1 (p160ROCK), Rho-associated protein kinase 2, Rho kinase 2, Rho-associated, coiled-coil-containing protein kinase 2, Rho-associated, coiled-coil-containing protein kinase II (ROCK-II), p164 ROCK-2
免疫原

Synthetic Peptide
细胞定位

Nucleus, Cytoplasm
Accession

Q13464, O75116
克隆号

S-1305-26
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu, Ms, Rt
预测反应种属
(反应种属缩写表)

Cz
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

ICFCM

ICC ?

WB

IP
稀释度

应用稀释度推荐种属

WB 1:1000 Hu, Ms, Rt
IP 1:50 Hu
ICC 1:500 Hu, Ms
ICFCM 1:50 Hu, Ms

应用	稀释度	推荐种属
WB	1:1000	Hu, Ms, Rt
IP	1:50	Hu
ICC	1:500	Hu, Ms
ICFCM	1:50	Hu, Ms

背景介绍

Rho-associated protein kinase (ROCK), also known as Rho-kinase, is a serine/threonine protein kinase that acts as a key downstream effector of the small GTPase RhoA. Once activated, ROCK can influence a multitude of cellular functions, such as the formation of actin filaments through the activation of LIM-kinase 2 (LIMK2), leading to increased actin polymerization. Additionally, ROCK is involved in smooth muscle contraction by inactivating myosin light chain phosphatase 1 (MYPT-1) through phosphorylation, thereby enhancing the phosphorylation of myosin light chains. ROCK has been implicated in a wide range of diseases, including pulmonary hypertension, coronary heart disease, respiratory disease, ocular disease, gastrointestinal disorders, and cancer. In the context of cancer, ROCK is believed to promote tumor progression by enhancing cell migration, invasion, and angiogenesis. Furthermore, ROCK signaling is also involved in the regulation of immune responses to viral infections and has been identified as a potential target for the development of novel antiviral therapeutics.

免疫印迹
- WB result of ROCK1+ROCK2 Recombinant Rabbit mAb
  Primary antibody: ROCK1+ROCK2 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: Jurkat whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 158 kDa
  Observed MW: 160 kDa
- WB result of ROCK1+ROCK2 Recombinant Rabbit mAb
  Primary antibody: ROCK1+ROCK2 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: NIH/3T3 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 158 kDa
  Observed MW: 160 kDa
  This blot was developed with high sensitivity substrate
- WB result of ROCK1+ROCK2 Recombinant Rabbit mAb
  Primary antibody: ROCK1+ROCK2 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: C6 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 158 kDa
  Observed MW: 160 kDa
流式分析
- Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) labelling ROCK1+ROCK2 antibody at 1/50 dilution (1 μg)/ (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
- Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized NIH/3T3 (Mouse embryonic fibroblast) labelling ROCK1+ROCK2 antibody at 1/50 dilution (1 μg)/ (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫沉淀
- ROCK1+ROCK2 Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating ROCK1+ROCK2 in 0.4 mg Jurkat whole cell lysate.
  Western blot was performed on the immunoprecipitate using ROCK1+ROCK2 Rabbit mAb at 1/1000 dilution.
  Secondary antibody (HRP) for IP was used at 1/1000 dilution.
  Lane 1: Jurkat whole cell lysate 20 µg (Input)
  Lane 2: ROCK1+ROCK2 Rabbit mAb IP in Jurkat whole cell lysate
  Lane 3: Rabbit monoclonal IgG IP in Jurkat whole cell lysate
  Predicted MW: 158 kDa
  Observed MW: 160 kDa
免疫细胞化学
- ICC shows positive staining in HeLa cells. Anti-ROCK1+ROCK2 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
- ICC shows positive staining in NIH/3T3 cells. Anti-ROCK1+ROCK2 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).