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CREB Recombinant Rabbit mAb (S-1115-39)

Cyclic AMP-responsive element-binding protein 1,CREB-1,cAMP-responsive element-binding protein 1,CREB1

货号： S0B0861

Datasheet COA
  • 价格： ￥260
  • 规格：
  • 数量：
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产品介绍 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    CREB

  • 分子别名

    Cyclic AMP-responsive element-binding protein 1; CREB-1; cAMP-responsive element-binding protein 1; CREB1

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Nucleus

  • Accession

    P16220

  • 克隆号

    S-1115-39

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 应用

    ICFCM, IHC-P, ICC, WB, IP

  • 反应种属 ?

    Hu, Ms

  • 预测反应种属
    (反应种属缩写表)

    Bv

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度
应用 稀释度
WB 1:1000
IP 1:50
IHC-P 1:500
ICC 1:500
ICFCM 1:500
背景介绍

  • CREB (cAMP response element-binding protein) is a transcription factor that plays a pivotal role in regulating gene expression, particularly in response to changes in intracellular cAMP levels. CREB proteins bind to cAMP response elements (CREs) on DNA, thereby controlling a variety of cellular functions, including cell proliferation, differentiation, metabolism, and the formation of learning and memory. In the field of neurobiology, CREB is especially important due to its close association with the molecular mechanisms of memory and learning. Studies have shown that the activation of CREB is related to long-term potentiation (LTP) and long-term depression (LTD) phenomena, which are considered to be the cellular basis for learning and memory. In addition to its role in the nervous system, CREB is also involved in regulating a variety of other physiological processes, including metabolic regulation, cell cycle control, muscle development, and reproductive functions. Abnormalities in CREB function are associated with a range of diseases, such as depression, neurodegenerative diseases, and certain types of cancer.

  • 免疫印迹

    • WB result of CERB Recombinant Rabbit mAb
      Primary antibody: CERB Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: HeLa whole cell lysate 20 µg
      Lane 2: A431 whole cell lysate 20 µg
      Lane 3: Jurkat whole cell lysate 20 µg
      Lane 4: K562 whole cell lysate 20 µg
      Lane 5: MCF7 whole cell lysate 20 µg
      Lane 6: MDA-MB-231 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 37 kDa
      Observed MW: 40 kDa

    • WB result of CERB Recombinant Rabbit mAb
      Primary antibody: CERB Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: C2C12 whole cell lysate 20 µg
      Lane 2: NIH/3T3 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 37 kDa
      Observed MW: 38 kDa

    • WB result of CERB Recombinant Rabbit mAb
      Primary antibody: CERB Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: PC-12 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 37 kDa
      Observed MW: 40 kDa

  • 流式分析

    • Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) labelling CREB antibody at 1/500 dilution (0.1 μg) / (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.

  • 免疫沉淀

    • CREB Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating CREB in 0.4 mg HeLa whole cell lysate.
      Western blot was performed on the immunoprecipitate using CREB Rabbit mAb at 1/1000 dilution.
      Secondary antibody (HRP) for IP was used at 1/1000 dilution.
      Lane 1: HeLa whole cell lysate 20 µg (Input)
      Lane 2: CREB Rabbit mAb IP in HeLa whole cell lysate
      Lane 3: Rabbit monoclonal IgG IP in HeLa whole cell lysate
      Predicted MW: 37 kDa
      Observed MW: 40 kDa

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human tonsil. Anti-CREB antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human colon. Anti-CREB antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human bladder cancer. Anti-CREB antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human breast cancer. Anti-CREB antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-CREB antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-CREB antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded rat stomach. Anti-CREB antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 免疫细胞化学

    • ICC shows positive staining in HeLa cells. Anti-CREB antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).

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