大包装询价 产品介绍 引用文献(0) 评论(0)
宿主来源
Rabbit抗原名称
MIF分子别名
Macrophage migration inhibitory factor, Glycosylation-inhibiting factor By (GIF), L-dopachrome isomerase, L-dopachrome tautomerase, Phenylpyruvate tautomerase, GLIF, MMIF免疫原
Recombinant Protein细胞定位
Secreted, CytoplasmAccession
P14174克隆号
S-1007-34抗体类型
Recombinant mAb抗体同种型
IgG应用
IHC-P, WB反应种属 ?
Hu, Ms, Rt纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 |
|---|---|
| WB | 1:1000 |
| IHC-P | 1:500 |
MIF (Macrophage Migration Inhibitory Factor) is a multifunctional cytokine that exhibits a wide range of biological activities beyond its original discovery as an inhibitor of macrophage migration. MIF is a key regulator of innate and adaptive immune responses. It promotes the release of pro-inflammatory cytokines like TNF-α, IL-1, IL-6, and IL-8, enhancing inflammation and recruiting immune cells to sites of injury or infection. However, MIF also plays a role in immune suppression, particularly in cancer, where it can inhibit the anti-tumor activity of macrophages and T cells. MIF promotes cell proliferation and survival through multiple mechanisms, including activation of the MAPK/ERK pathway, inhibition of p53-mediated apoptosis, and upregulation of anti-apoptotic proteins. These effects contribute to the growth and progression of various cancers. MIF is a critical regulator of glucose homeostasis, acting as a counter-regulatory hormone to insulin. It inhibits insulin-mediated glucose uptake in adipocytes and muscle cells, promoting hyperglycemia in response to stress or inflammation. This function of MIF has implications for the development of insulin resistance and type 2 diabetes. MIF is also involved in the regulation of pituitary hormone secretion, particularly prolactin and ACTH. It stimulates the release of these hormones, which can have wide-ranging effects on reproductive function, stress response, and metabolism.
免疫印迹
WB result of MIF Recombinant Rabbit mAb
Primary antibody: MIF Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Jurkat whole cell lysate 20 µg
Lane 2: THP-1 whole cell lysate 20 µg
Lane 3: U-87 MG whole cell lysate 20 µg
Lane 4: 293T whole cell lysate 20 µg
Lane 5: Molt-4 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 12 kDa
Observed MW: 12 kDaWB result of MIF Recombinant Rabbit mAb
Primary antibody: MIF Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Neuro-2a whole cell lysate 20 µg
Lane 2: NIH/3T3 whole cell lysate 20 µg
Lane 3: RAW264.7 whole cell lysate 20 µg
Lane 4: mouse brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 12 kDa
Observed MW: 12 kDaWB result of MIF Recombinant Rabbit mAb
Primary antibody: MIF Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 12 kDa
Observed MW: 13 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti- MIF antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human kidney. Anti- MIF antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human prostatic hyperplasia. Anti- MIF antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human prostatic cancer. Anti- MIF antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti- MIF antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti- MIF antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti- MIF antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
引用文献(0)
评论(0)