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MIF Recombinant Rabbit mAb (S-1007-34)

Macrophage migration inhibitory factor,Glycosylation-inhibiting factor By (GIF),L-dopachrome isomerase,L-dopachrome tautomerase,Phenylpyruvate tautomerase,GLIF,MMIF

价格 ￥600.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B0858
规格
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产品介绍 FAQs 引用文献(2) 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    MIF

  • 分子别名

    Macrophage migration inhibitory factor, Glycosylation-inhibiting factor By (GIF), L-dopachrome isomerase, L-dopachrome tautomerase, Phenylpyruvate tautomerase, GLIF, MMIF

  • 免疫原

    Recombinant Protein

  • 细胞定位

    Cytoplasm, Secreted

  • Accession

    P14174

  • 克隆号

    S-1007-34

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 应用

    IHC-P ? ,
    WB

  • 反应种属 ?

    Hu, Ms, Rt

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度
应用 稀释度
WB 1:1000
IHC-P 1:500
背景介绍

  • MIF (Macrophage Migration Inhibitory Factor) is a multifunctional cytokine that exhibits a wide range of biological activities beyond its original discovery as an inhibitor of macrophage migration. MIF is a key regulator of innate and adaptive immune responses. It promotes the release of pro-inflammatory cytokines like TNF-α, IL-1, IL-6, and IL-8, enhancing inflammation and recruiting immune cells to sites of injury or infection. However, MIF also plays a role in immune suppression, particularly in cancer, where it can inhibit the anti-tumor activity of macrophages and T cells. MIF promotes cell proliferation and survival through multiple mechanisms, including activation of the MAPK/ERK pathway, inhibition of p53-mediated apoptosis, and upregulation of anti-apoptotic proteins. These effects contribute to the growth and progression of various cancers. MIF is a critical regulator of glucose homeostasis, acting as a counter-regulatory hormone to insulin. It inhibits insulin-mediated glucose uptake in adipocytes and muscle cells, promoting hyperglycemia in response to stress or inflammation. This function of MIF has implications for the development of insulin resistance and type 2 diabetes. MIF is also involved in the regulation of pituitary hormone secretion, particularly prolactin and ACTH. It stimulates the release of these hormones, which can have wide-ranging effects on reproductive function, stress response, and metabolism.

  • 免疫印迹

    • WB result of MIF Recombinant Rabbit mAb
      Primary antibody: MIF Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: Jurkat whole cell lysate 20 µg
      Lane 2: THP-1 whole cell lysate 20 µg
      Lane 3: U-87 MG whole cell lysate 20 µg
      Lane 4: 293T whole cell lysate 20 µg
      Lane 5: Molt-4 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 12 kDa
      Observed MW: 12 kDa

    • WB result of MIF Recombinant Rabbit mAb
      Primary antibody: MIF Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: Neuro-2a whole cell lysate 20 µg
      Lane 2: NIH/3T3 whole cell lysate 20 µg
      Lane 3: RAW264.7 whole cell lysate 20 µg
      Lane 4: mouse brain lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 12 kDa
      Observed MW: 12 kDa

    • WB result of MIF Recombinant Rabbit mAb
      Primary antibody: MIF Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: rat brain lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 12 kDa
      Observed MW: 13 kDa

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti- MIF antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human kidney. Anti- MIF antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human prostatic hyperplasia. Anti- MIF antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human prostatic cancer. Anti- MIF antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti- MIF antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti- MIF antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti- MIF antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 斯达特公司的抗体，可以回收利用几次？

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