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MEK1/2 Recombinant Rabbit mAb (S-834-101)

Dual specificity mitogen-activated protein kinase kinase 1,MAP kinase kinase 1,MAPKK 1,MKK1,ERK activator kinase 1,MAPK/ERK kinase 1

浏览次数：94

货号： S0B0857

Datasheet COA

价格： ￥600
规格：
10μl25μl100μl1ml
数量：

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产品规格

宿主来源

Rabbit
抗原名称

MEK1/2
分子别名

Dual specificity mitogen-activated protein kinase kinase 1; MAP kinase kinase 1; MAPKK 1; MKK1; ERK activator kinase 1; MAPK/ERK kinase 1
免疫原

Recombinant Protein
细胞定位

Cytoskeleton, Cytoplasm, Nucleus, Membrane
Accession

Q02750
克隆号

S-834-101
抗体类型

Recombinant mAb
抗体同种型

IgG
应用

ICFCM, IHC-P, ICC, WB, IP
反应种属 ?

Hu, Ms, Rt
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度

应用	稀释度
WB	1:5000
IP	1:50
IHC-P	1:250
ICC	1:500
ICFCM	1:500

背景介绍

MEK1/2 (Mitogen-Activated Protein Kinase Kinase 1 and 2) are crucial components within the MAPK (Mitogen-Activated Protein Kinase) signaling pathway, belonging to the protein kinase family. They are activated by upstream kinases such as RAF, which then phosphorylate and activate downstream ERK (Extracellular Signal-Regulated Kinase) protein kinases, thereby promoting cellular processes like growth, differentiation, and proliferation. In many cancers, MEK1/2 activity is significantly elevated, enabling tumor cells to proliferate and grow unchecked. Therefore, inhibiting the MEK1/2 signaling pathway is considered a vital anticancer strategy.

免疫印迹
- WB result of MEK1/2 Recombinant Rabbit mAb
  Primary antibody: MEK1/2 Recombinant Rabbit mAb at 1/5000 dilution
  Lane 1: HeLa whole cell lysate 20 µg
  Lane 2: A431 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 43 kDa
  Observed MW: 45 kDa
- WB result of MEK1/2 Recombinant Rabbit mAb
  Primary antibody: MEK1/2 Recombinant Rabbit mAb at 1/5000 dilution
  Lane 1: NIH/3T3 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 43 kDa
  Observed MW: 45 kDa
- WB result of MEK1/2 Recombinant Rabbit mAb
  Primary antibody: MEK1/2 Recombinant Rabbit mAb at 1/5000 dilution
  Lane 1: PC-12 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 43 kDa
  Observed MW: 45 kDa
流式分析
- Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized NIH/3T3 (Mouse embryonic fibroblast) labelling MEK1/2 antibody at 1/500 dilution (0.1 μg)/ (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫沉淀
- MEK1/2 Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating MEK1/2 in 0.4 mg HeLa whole cell lysate.
  Western blot was performed on the immunoprecipitate using MEK1/2 Rabbit mAb at 1/1000 dilution.
  Secondary antibody (HRP) for IP was used at 1/1000 dilution.
  Lane 1: HeLa whole cell lysate 20 µg (Input)
  Lane 2: MEK1/2 Rabbit mAb IP in HeLa whole cell lysate
  Lane 3: Rabbit monoclonal IgG IP in HeLa whole cell lysate
  Predicted MW: 43 kDa
  Observed MW: 45 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human colon. Anti-MEK1/2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human kidney. Anti-MEK1/2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- Negative control: IHC shows negative staining in paraffin-embedded human liver. Anti-MEK1/2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human colon cancer. Anti-MEK1/2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human breast cancer. Anti-MEK1/2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
- ICC shows positive staining in HeLa cells. Anti-MEK1/2 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
- ICC shows positive staining in NIH/3T3 cells. Anti-MEK1/2 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).