Phospho-Stat5 (Tyr694) Recombinant Rabbit mAb (S-1183-58)

Signal transducer and activator of transcription 5A,STAT5A

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0851

规格

25μl100μl1ml

数量

产品介绍

产品介绍 FAQs 评论(0)

产品规格

宿主来源

Rabbit
抗原名称

Phospho-Stat5 (Tyr694)
分子别名

Signal transducer and activator of transcription 5A; STAT5A
免疫原

Synthetic Peptide
细胞定位

Nucleus
Accession

P42229
克隆号

S-1183-58
抗体类型

Recombinant mAb
抗体同种型

IgG
翻译后修饰类型

磷酸化
反应种属 ?

Hu, Ms, Rt
预测反应种属
(反应种属缩写表)

Bv, Sh
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

ICFCM ,
ICC ? ,
WB ,
IP
稀释度

应用稀释度

Dot Blot 1:1000
WB 1:1000
IP 1:50
ICC 1:100
ICFCM 1:200

应用	稀释度
Dot Blot	1:1000
WB	1:1000
IP	1:50
ICC	1:100
ICFCM	1:200

背景介绍

Stat5, which stands for Signal Transducer and Activator of Transcription 5, is a transcription factor that plays a pivotal role in cellular signaling pathways. It is a key player in cellular signaling, particularly in response to cytokine receptors such as IL-2, IL-3, IL-5, and GM-CSF. Upon binding of these cytokines to their respective receptors, a cascade of signaling events is triggered, ultimately leading to the phosphorylation of Stat5 (e.g., at Tyr694). The phosphorylated Stat5 forms dimers and translocates to the nucleus, where it acts as a transcription factor to regulate the expression of target genes.

免疫印迹
- WB result of Phospho-Stat5 (Tyr694) Recombinant Rabbit mAb
  Primary antibody: Phospho-Stat5 (Tyr694) Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: untreated A431 whole cell lysate 20 µg
  Lane 2: A431 treated with 100 ng/ml EGF for 5 minutes whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 91 kDa
  Observed MW: 90 kDa
流式分析
- Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized human peripheral blood T lymphocytes, treated with CD3, CD28 beads (Right panel) or untreated (Left panel), labeling Phospho-Stat5 (Tyr694) antibody at 1/200 dilution (0.25 μg)/ (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫沉淀
- Phospho-Stat5 (Tyr694) Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating Phospho-Stat5 (Tyr694) in 0.4 mg A431 treated with 100 ng/ml EGF for 5 minutes whole cell lysate.
  Western blot was performed on the immunoprecipitate using Phospho-Stat5 (Tyr694) Rabbit mAb at 1/1000 dilution.
  Secondary antibody (HRP) for IP was used at 1/1000 dilution.
  Lane 1: A431 treated with 100 ng/ml EGF for 5 minutes whole cell lysate 20 µg (Input)
  Lane 2: Phospho-Stat5 (Tyr694) Rabbit mAb IP in A431 treated with 100 ng/ml EGF for 5 minutes whole cell lysate
  Lane 3: Rabbit monoclonal IgG IP in A431 treated with 100 ng/ml EGF for 5 minutes whole cell lysate
  Predicted MW: 91 kDa
  Observed MW: 90 kDa
斑点杂交
- Dot blot result of Phospho-Stat5 (Tyr694) Recombinant Rabbit mAb
  Lane 1: Phospho-Stat5 (Tyr694) peptide
  Lane 2: Stat5 unmodified peptide
  Primary antibody: Phospho-Stat5 (Tyr694) Recombinant Rabbit mAb at 1/1000 dilution
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
免疫细胞化学
- ICC analysis of A431 cells treated with EGF (100ng/mL,5min) (top panel) and A431 cells untreated with EGF (100ng/mL,5min) (below panel). Anti-Phospho-Stat5 (Tyr694) antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).