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Phospho-PLCγ1 (Tyr783) Recombinant Rabbit mAb (S-1184-12)

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1,PLC-148,Phosphoinositide phospholipase C-gamma-1,Phospholipase C-II (PLC-II),Phospholipase C-gamma-1 (PLC-gamma-1),PLCG1

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0850

规格

25μl100μl1ml

数量

产品介绍

产品介绍 FAQs 评论(0)

产品规格

宿主来源

Rabbit
抗原名称

Phospho-PLCγ1 (Tyr783)
分子别名

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1, PLC-148, Phosphoinositide phospholipase C-gamma-1, Phospholipase C-II (PLC-II), Phospholipase C-gamma-1 (PLC-gamma-1), PLCG1
免疫原

Synthetic Peptide
细胞定位

Cytoplasm
Accession

P19174
克隆号

S-1184-12
抗体类型

Recombinant mAb
抗体同种型

IgG
翻译后修饰类型

磷酸化
反应种属 ?

Hu
预测反应种属
(反应种属缩写表)

Ms, Bv, Rt
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

ICC ?

WB
稀释度

应用稀释度

Dot Blot 1:1000
WB 1:1000
ICC 1:500

应用	稀释度
Dot Blot	1:1000
WB	1:1000
ICC	1:500

背景介绍

PLCG1, or Phospholipase C Gamma 1, is a crucial enzyme protein involved in cellular signaling, differentiation, proliferation, and various other biological processes. it is crucial in receptor-mediated tyrosine kinase activator signaling within cells. Receptor tyrosine kinases interact with SRC domains to enzymatically activate PLCγ1 via phosphorylation at tyrosine 783. Upon activation PLCG1 leads to the translocation of Ras guanine nucleotide exchange factor RasGRP1 to the Golgi apparatus, activating Ras. PLCG1 is also a primary substrate of heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinases.

免疫印迹
- WB result of Phospho-PLCγ1 (Tyr783) Recombinant Rabbit mAb
  Primary antibody: Phospho-PLCγ1 (Tyr783) Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: untreated Jurkat whole cell lysate 20 µg
  Lane 2: Jurkat treated with 50 mM pervanadate for 5 minutes whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 149 kDa
  Observed MW: 150 kDa
斑点杂交
- Dot blot result of Phospho-PLCγ1 (Tyr783) Recombinant Rabbit mAb
  Lane 1: Phospho-PLCγ1 (Tyr783) peptide
  Lane 2: PLCγ1 unmodified peptide
  Primary antibody: Phospho-PLCγ1 (Tyr783) Recombinant Rabbit mAb at 1/1000 dilution
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
免疫细胞化学
- ICC analysis of Jurkat cells treated with pervanadate (50mM, 5mins) (top panel) and Jurkat cells untreated with pervanadate (50mM, 5mins) (below panel). Anti- Phospho-PLCγ1 (Tyr783) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).