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宿主来源
Rabbit抗原名称
FABP4分子别名
Adipocyte fatty acid-binding protein, Adipocyte lipid-binding protein (ALBP), Adipocyte-type fatty acid-binding protein (A-FABP; AFABP), Fatty acid-binding protein 4免疫原
Synthetic Peptide细胞定位
Nucleus, CytoplasmAccession
P15090克隆号
S-1360-58抗体类型
Recombinant mAb抗体同种型
IgG应用
IHC-P, WB, IF反应种属 ?
Hu, Ms, Rt预测反应种属
(反应种属缩写表)Pg纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 |
|---|---|
| WB | 1:1000 |
| IHC-P | 1:1000 |
| IF | 1:1000 |
FABP4, also known as Adipocyte Fatty Acid Binding Protein (A-FABP) or aP2, is a small cytoplasmic protein belonging to the family of fatty acid-binding proteins (FABPs). It is widely expressed in various tissues, including adipose tissue, macrophages, small intestine, heart, and breast, with particularly high levels in adipocytes and macrophages. The primary function of FABP4 is to serve as an intracellular transporter of fatty acids. It facilitates the uptake, transport, and metabolism of fatty acids by binding to long-chain fatty acids (LCFAs) to form stable complexes. These complexes then shuttle the fatty acids to various intracellular compartments such as mitochondria, endoplasmic reticulum, or peroxisomes for processes like β-oxidation, esterification, or phospholipid synthesis. In addition to its role in fatty acid metabolism, FABP4 is also involved in regulating adipocyte differentiation and insulin sensitivity. Moreover, it plays a significant part in inflammatory responses and immune modulation.
免疫印迹
WB result of FABP4 Recombinant Rabbit mAb
Primary antibody: FABP4 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse adipose lysate 20 µg
Lane 2: mouse heart lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 15 kDa
Observed MW: 13 kDaWB result of FABP4 Recombinant Rabbit mAb
Primary antibody: FABP4 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat heart lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 15 kDa
Observed MW: 13 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human breast. Anti-FABP4 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cardiac muscle. Anti-FABP4 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human skeletal muscle. Anti-FABP4 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human placenta. Anti-FABP4 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-FABP4 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫荧光
IF shows positive staining in paraffin-embedded human breast. Anti-FABP4 antibody was used at 1/1000 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
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