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Helios Recombinant Rabbit mAb (S-1299-31)

Zinc finger protein Helios,Ikaros family zinc finger protein 2,IKZF2,ZNFN1A2

价格 ￥600.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B0845
规格
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产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    Helios

  • 分子别名

    Zinc finger protein Helios, Ikaros family zinc finger protein 2, IKZF2, ZNFN1A2

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Nucleus

  • Accession

    Q9UKS7

  • 克隆号

    S-1299-31

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 应用

    ICFCM ,
    IHC-P ? ,
    ICC ? ,
    WB

  • 反应种属 ?

    Hu, Ms, Rt

  • 纯化方式

    Protein A

  • 浓度

    2 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度
应用 稀释度
WB 1:4000
IHC-P 1:500
ICC 1:2000
ICFCM 1:200
背景介绍

  • The Helios protein belongs to the Ikaros family of zinc finger proteins, which are hematopoietic-specific transcription factors involved in the regulation of lymphocyte development. The Helios protein serves as an epigenetic regulator, selectively expressing some genes while suppressing others. It influences gene expression by modulating chromatin structure, thereby controlling DNA accessibility. This regulatory mechanism is vital for normal cell growth and differentiation. The Helios protein exhibits a unique dual role in leukemia. Its deficiency has been linked to the development of acute B-lymphoblastic leukemia (B-ALL), while its overexpression promotes acute myeloid leukemia (AML).

  • 免疫印迹

    • WB result of Helios Recombinant Rabbit mAb
      Primary antibody: Helios Recombinant Rabbit mAb at 1/4000 dilution
      Lane 1: Jurkat whole cell lysate 20 µg
      Lane 2: Raji whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 60 kDa
      Observed MW: 55, 85 kDa

    • WB result of Helios Recombinant Rabbit mAb
      Primary antibody: Helios Recombinant Rabbit mAb at 1/4000 dilution
      Lane 1: EL4 whole cell lysate 20 µg
      Lane 2: mouse thymus lysate 20 µg
      Lane 3: mouse spleen lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 60 kDa
      Observed MW: 55, 63~70, 85 kDa

    • WB result of Helios Recombinant Rabbit mAb
      Primary antibody: Helios Recombinant Rabbit mAb at 1/4000 dilution
      Lane 1: rat thymus lysate 20 µg
      Lane 3: rat spleen lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 60 kDa
      Observed MW: 55, 85 kDa

  • 流式分析

    • Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized Jurkat (Human T cell leukemia T lymphocyte) labelling Helios antibody at 1/200 dilution (1 μg)/ (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human thymus. Anti-Helios antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-Helios antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-Helios antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human thyroid carcinoma. Anti-Helios antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 免疫细胞化学

    • ICC shows positive staining in Jurkat cells. Anti- Helios antibody was used at 1/2000 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).

  • 斯达特公司的抗体，可以回收利用几次？

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