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宿主来源
Rabbit抗原名称
Pembrolizumab分子别名
Keytruda克隆号
SDT-825-21-1抗体类型
Recombinant mAb抗体同种型
IgG纯化方式
Protein A标记
Unconjugated性状
Lyophilized Powder缓冲体系
Lyophilized with PBS pH7.4, 0.03% Proclin 300
溶解方法
Reconstitute the lyophilized powder with deionized water to a final concentration of 0.5 mg/ml.储存条件
The lyophilized product remains stable up to 1 year at -20 °C from date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C . -80 °C for longer storage. Avoid repeated freezing and thawing cycles.
应用
Competitive ELISA
PK study
ELISA
稀释度
应用 稀释度 ELISA 0.005-0.05 µg/ml
Pembrolizumab, sold under the brand name Keytruda, is a humanized antibody used in cancer immunotherapy that treats melanoma, lung cancer, head and neck cancer, Hodgkin lymphoma, stomach cancer, cervical cancer, and certain types of breast cancer. It is administered by slow intravenous injection. It is an IgG4 isotype antibody that blocks a protective mechanism of cancer cells, allowing the immune system to destroy them. It targets the programmed cell death protein 1 (PD-1) receptor of lymphocytes by blocking its interaction with PD-L1, resulting in the activation of T-cell-mediated immune responses against tumor cells.
ELISA
ELISA binding of Anti-Pembrolizumab Recombinant Rabbit mAb (SDT-825-21-1) with Pembrolizumab. While the antibody does not recognize the human IgG4 control and human total IgG (data not shown).
Coating antigen: Pembrolizumab, 0.2 µg/ml.
Anti-Pembrolizumab Recombinant Rabbit mAb (SDT-825-21-1) (S0B0843) dilutions start from 500 ng/ml.
EC50= 3.983 ng/ml.
竞争ELISA
Anti-Pembrolizumab Recombinant Rabbit mAb (SDT-825-21-1) (S0B0843) blocks Pembrolizumab binding with PD-1 Fc Chimera Protein, Human (UA010002, STARTER).
Coating antigen: Pembrolizumab, 1 µg/ml
PD-1-Fc-biotin final concentration: 5 ng/ml
Anti-Pembrolizumab Recombinant Rabbit mAb (SDT-825-21-1) (S0B0843) dilutions start from 40 μg/ml.
IC50= 344 ng/ml.
配对推荐
Standard curve of Pembrolizumab Sandwich ELISA. The Pembrolizumab Sandwich ELISA assay is developed by using Anti-Pembrolizumab Recombinant Rabbit mAb (SDT-825-61) (S0B0842) and Anti-Pembrolizumab Recombinant Rabbit mAb (SDT-825-21-1) (S0B0843) as the capture and detection antibodies, respectively.
In this ELISA assay, Anti-Pembrolizumab Recombinant Rabbit mAb (SDT-825-21-1) (S0B0843) was labeled with Biotin.
The sensitivity of detecting Pembrolizumab is up to 29 pg/ml.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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