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SMAD1 Rabbit Polyclonal antibody

Mothers against decapentaplegic homolog 1,MAD homolog 1,Mothers against DPP homolog 1,JV4-1,Mad-related protein 1,SMAD family member 1,Transforming growth factor-beta-signaling protein 1 (BSP-1),BSP1,MADH1,MADR1

价格 ￥400.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B0835
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产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    SMAD1

  • 分子别名

    Mothers against decapentaplegic homolog 1; MAD homolog 1; Mothers against DPP homolog 1; JV4-1; Mad-related protein 1; SMAD family member 1; Transforming growth factor-beta-signaling protein 1 (BSP-1); BSP1; MADH1; MADR1

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Cytoplasm, Nucleus

  • Accession

    Q15797

  • 抗体类型

    Polyclonal antibody

  • 抗体同种型

    IgG

  • 应用

    ICFCM ,
    IHC-P ? ,
    ICC ? ,
    WB ,
    IP

  • 反应种属 ?

    Hu, Ms, Rt

  • 预测反应种属
    (反应种属缩写表)

    Av, Bv

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度
应用 稀释度
WB 1:1000
IP 1:50
IHC-P 1:250
ICC 1:500
ICFCM 1:500
背景介绍

  • Smad1, or Smad Homolog 1, is a vital protein involved in cellular signal transduction, particularly within the TGF-β (Transforming Growth Factor-β) signaling pathway. Smad1 serves as a key transcription factor in the TGF-β signaling pathway, facilitating the transmission of extracellular signals from the cell membrane to the nucleus, where it regulates the transcription of target genes. Research indicates that Smad1 plays a crucial role in bone development and metabolism. For instance, BMP9 activates the Smad1 signaling pathway, inhibiting osteoblast senescence and thereby reducing age-related bone loss in mice. The role of Smad1 in tumorigenesis is also gaining attention. Studies have found that Atm-mediated phosphorylation of Smad1 is important in the DNA damage response and tumorigenesis. In ophthalmology research, Smad1 has been shown to have a protective role in mouse refractive development and the formation of form-deprivation myopia.

  • 免疫印迹

    • WB result of SMAD1 Rabbit pAb
      Primary antibody: SMAD1 Rabbit pAb at 1/1000 dilution
      Lane 1: HeLa whole cell lysate 20 µg
      Lane 2: HEK-293 whole cell lysate 20 µg
      Lane 3: HT-1080 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 52 kDa
      Observed MW: 60 kDa

    • WB result of SMAD1 Rabbit pAb
      Primary antibody: SMAD1 Rabbit pAb at 1/1000 dilution
      Lane 1: NIH/3T3 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 52 kDa
      Observed MW: 60 kDa

  • 流式分析

    • Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) labelling SMAD1 antibody at 1/500 dilution (0.1 μg)/ (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.

  • 免疫沉淀

    • SMAD1 Rabbit pAb at 1/50 dilution (1 µg) immunoprecipitating SMAD1 in 0.4 mg HeLa whole cell lysate.
      Western blot was performed on the immunoprecipitate using SMAD1 Rabbit pAb at 1/1000 dilution.
      Secondary antibody (HRP) for IP was used at 1/1000 dilution.
      Lane 1: HeLa whole cell lysate 40 µg (Input)
      Lane 2: SMAD1 Rabbit pAb IP in HeLa whole cell lysate
      Lane 3: Rabbit monoclonal IgG IP in HeLa whole cell lysate
      Predicted MW: 52 kDa
      Observed MW: 60 kDa

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human tonsil. Anti-SMAD1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human stomach. Anti-SMAD1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-SMAD1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded rat colon. Anti-SMAD1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 免疫细胞化学

    • ICC shows positive staining in HeLa cells. Anti-SMAD1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).

  • 斯达特公司的抗体，可以回收利用几次？

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