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Rabbit抗原名称
Vesicular Glutamate Transporter 1 (VGLUT1)分子别名
Brain-specific Na(+)-dependent inorganic phosphate cotransporter, Solute carrier family 17 member 7, SLC17A7, BNPI免疫原
Synthetic Peptide细胞定位
Cell membrane, SynapseAccession
Q9P2U7克隆号
S-1118-58抗体类型
Recombinant mAb抗体同种型
IgG应用
IHC-P, WB, IF反应种属 ?
Hu, Ms, Rt预测反应种属
(反应种属缩写表)Bv纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 |
|---|---|
| WB | 1:1000 |
| IHC-P | 1:1000 |
| IF | 1:500 |
Vesicular glutamate transporter 1 (VGLUT1) is a vesicle-bound, sodium-dependent phosphate transporter that is specifically expressed in the neuron-rich regions of the brain. It is preferentially associated with the membranes of synaptic vesicles and functions in glutamate transport. The protein shares 82% identity with the differentiation-associated Na-dependent inorganic phosphate cotransporter and they appear to form a distinct class within the Na+/Pi cotransporter family.
免疫印迹
WB result of Vesicular Glutamate Transporter 1 (VGLUT1) Recombinant Rabbit mAb
Primary antibody: Vesicular Glutamate Transporter 1 (VGLUT1) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: unboiled mouse liver lysate 20 µg
Lane 2: unboiled mouse brain lysate 20 µg
Negative control: unboiled mouse liver lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 62 kDa
Observed MW: 55 kDaWB result of Vesicular Glutamate Transporter 1 (VGLUT1) Recombinant Rabbit mAb
Primary antibody: Vesicular Glutamate Transporter 1 (VGLUT1) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: unboiled rat liver lysate 20 µg
Lane 2: unboiled rat brain lysate 20 µg
Negative control: unboiled rat liver lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 62 kDa
Observed MW: 55 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-Vesicular Glutamate Transporter 1 (VGLUT1) antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cerebellum. Anti-Vesicular Glutamate Transporter 1 (VGLUT1) antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human liver. Anti-Vesicular Glutamate Transporter 1 (VGLUT1) antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-Vesicular Glutamate Transporter 1 (VGLUT1) antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebellum. Anti-Vesicular Glutamate Transporter 1 (VGLUT1) antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded mouse liver. Anti-Vesicular Glutamate Transporter 1 (VGLUT1) antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-Vesicular Glutamate Transporter 1 (VGLUT1) antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded rat testis. Anti-Vesicular Glutamate Transporter 1 (VGLUT1) antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫荧光
IF shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-Vesicular Glutamate Transporter 1 (VGLUT1) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded mouse cerebellum. Anti-Vesicular Glutamate Transporter 1 (VGLUT1) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
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