Phospho-SRC (Tyr419) Recombinant Rabbit mAb (S-1182-5)

Proto-oncogene tyrosine-protein kinase Src,Proto-oncogene c-Src,pp60c-src (p60-Src)

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0832

规格

25μl100μl1ml

数量

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产品规格

宿主来源

Rabbit
抗原名称

Phospho-SRC (Tyr419)
分子别名

Proto-oncogene tyrosine-protein kinase Src, Proto-oncogene c-Src, pp60c-src (p60-Src)
免疫原

Synthetic Peptide
细胞定位

Cell membrane, Cytoplasm, Nucleus
Accession

P12931
克隆号

S-1182-5
抗体类型

Recombinant mAb
抗体同种型

IgG
翻译后修饰类型

磷酸化
应用

ICC ? ,
WB ,
IP
反应种属 ?

Hu, Rt
预测反应种属
(反应种属缩写表)

Zf, Fs, Rt, Ms, Av, Dg, Xe, Bv, Pg
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度

应用	稀释度
Dot Blot	1:1000
WB	1:1000
IP	1:50
ICC	1:100

背景介绍

Proto-oncogene tyrosine-protein kinase Src, also known as proto-oncogene c-Src, or simply c-Src belongs to a family of Src family kinases. c-Src phosphorylates specific tyrosine residues in other tyrosine kinases. It plays a role in the regulation of embryonic development and cell growth. An elevated level of activity of c-Src is suggested to be linked to cancer progression by promoting other signals. Mutations in c-Src could be involved in the malignant progression of colon cancer. The activation of c-Src causes the dephosphorylation of the tyrosine 527. This induces long-range allostery via protein domain dynamics, causing the structure to be destabilized, resulting in the opening up of the SH3, SH2 and kinase domains and the autophosphorylation of the residue tyrosine 419.

免疫印迹
- WB result of Phospho-SRC (Tyr419) Recombinant Rabbit mAb
  Primary antibody: Phospho-SRC (Tyr419) Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: untreated A431 whole cell lysate 20 µg
  Lane 2: A431 treated with 50 mM pervanadate for 5 minutes whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 60 kDa
  Observed MW: 60 kDa
- WB result of Phospho-SRC (Tyr419) Recombinant Rabbit mAb
  Primary antibody: Phospho-SRC (Tyr419) Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: Untreated C6 whole cell lysate 20 µg
  Lane 2: C6 treated with pervanadate(10mM, 20min) whole cell lysate 20 µg
  Negative control: Untreated C6 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 60 kDa
  Observed MW: 60kDa
免疫沉淀
- Phospho-SRC (Tyr419) Recombinan Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating Phospho-SRC (Tyr419) in 0.4 mg A431 treated with 50mM pervanadate for 5 minutes whole cell lysate.
  Western blot was performed on the immunoprecipitate using Phospho-SRC (Tyr419) Recombinan Rabbit mAb at 1/1000 dilution.
  Secondary antibody (HRP) for IP was used at 1/1000 dilution.
  Lane 1: A431 treated with 50mM pervanadate for 5 minutes whole cell lysate 20 µg (Input)
  Lane 2: Phospho-SRC (Tyr419) Rabbit mAb IP in A431 treated with 50mM pervanadate for 5 minutes whole cell lysate
  Lane 3: Rabbit monoclonal IgG IP in A431 treated with 50mM pervanadate for 5 minutes whole cell lysate
  Predicted MW: 60 kDa
  Observed MW: 60kDa
斑点杂交
- Dot blot result of Phospho-SRC (Tyr419) Recombinant Rabbit mAb
  Lane 1: Phospho-SRC (Tyr419) peptide
  Lane 2: SRC unmodified peptide
  Primary antibody: Phospho-SRC (Tyr419) Recombinant Rabbit mAb at 1/1000 dilution
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
免疫细胞化学
- ICC analysis of A431 cells treated with 50mM pervanadate for 5 minutes (top panel) and untreated A431 cells (below panel). Anti- Phospho-SRC (Tyr419) antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).