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Rabbit抗原名称
Phospho-SRC (Tyr419)分子别名
Proto-oncogene tyrosine-protein kinase Src, Proto-oncogene c-Src, pp60c-src (p60-Src)免疫原
Synthetic Peptide细胞定位
Nucleus, Cell membrane, CytoplasmAccession
P12931克隆号
S-1182-5抗体类型
Recombinant mAb抗体同种型
IgG翻译后修饰类型
磷酸化反应种属 ?
Hu, Rt预测反应种属
(反应种属缩写表)Zf, Fs, Rt, Ms, Av, Dg, Xe, Bv, Pg纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
ICC ?
WB
IP
稀释度
应用 稀释度 推荐种属 Dot Blot 1:1000 WB 1:1000 Hu, Rt IP 1:50 Hu ICC 1:100 Hu
Proto-oncogene tyrosine-protein kinase Src, also known as proto-oncogene c-Src, or simply c-Src belongs to a family of Src family kinases. c-Src phosphorylates specific tyrosine residues in other tyrosine kinases. It plays a role in the regulation of embryonic development and cell growth. An elevated level of activity of c-Src is suggested to be linked to cancer progression by promoting other signals. Mutations in c-Src could be involved in the malignant progression of colon cancer. The activation of c-Src causes the dephosphorylation of the tyrosine 527. This induces long-range allostery via protein domain dynamics, causing the structure to be destabilized, resulting in the opening up of the SH3, SH2 and kinase domains and the autophosphorylation of the residue tyrosine 419.
免疫印迹
WB result of Phospho-SRC (Tyr419) Recombinant Rabbit mAb
Primary antibody: Phospho-SRC (Tyr419) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated A431 whole cell lysate 20 µg
Lane 2: A431 treated with 50 mM pervanadate for 5 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 60 kDa
Observed MW: 60 kDaWB result of Phospho-SRC (Tyr419) Recombinant Rabbit mAb
Primary antibody: Phospho-SRC (Tyr419) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Untreated C6 whole cell lysate 20 µg
Lane 2: C6 treated with pervanadate(10mM, 20min) whole cell lysate 20 µg
Negative control: Untreated C6 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 60 kDa
Observed MW: 60kDa
免疫沉淀
Phospho-SRC (Tyr419) Recombinan Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating Phospho-SRC (Tyr419) in 0.4 mg A431 treated with 50mM pervanadate for 5 minutes whole cell lysate.
Western blot was performed on the immunoprecipitate using Phospho-SRC (Tyr419) Recombinan Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/1000 dilution.
Lane 1: A431 treated with 50mM pervanadate for 5 minutes whole cell lysate 20 µg (Input)
Lane 2: Phospho-SRC (Tyr419) Rabbit mAb IP in A431 treated with 50mM pervanadate for 5 minutes whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in A431 treated with 50mM pervanadate for 5 minutes whole cell lysate
Predicted MW: 60 kDa
Observed MW: 60kDa
斑点杂交
Dot blot result of Phospho-SRC (Tyr419) Recombinant Rabbit mAb
Lane 1: Phospho-SRC (Tyr419) peptide
Lane 2: SRC unmodified peptide
Primary antibody: Phospho-SRC (Tyr419) Recombinant Rabbit mAb at 1/1000 dilution
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
免疫细胞化学
ICC analysis of A431 cells treated with 50mM pervanadate for 5 minutes (top panel) and untreated A431 cells (below panel). Anti- Phospho-SRC (Tyr419) antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
我们推荐客户使用TPST+5%脱脂奶粉来稀释一抗，进行封闭。 虽然BSA被推荐为WB检测磷酸化蛋白的常用封闭剂，但是脱脂奶粉获取更加方便，覆盖更广泛的非特异性结合位点，在一抗性能优越的前提下，使用脱脂奶粉封闭性价比更高
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