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Rabbit抗原名称
Phospho-SRC (Tyr419)分子别名
Proto-oncogene tyrosine-protein kinase Src; Proto-oncogene c-Src; pp60c-src (p60-Src)免疫原
Synthetic Peptide细胞定位
Cytoplasm, Nucleus, Cell membraneAccession
P12931克隆号
S-1182-5抗体类型
Recombinant mAb抗体同种型
IgG应用
WB, IP反应种属 ?
Hu预测反应种属
(反应种属缩写表)Zf, Fs, Rt, Ms, Av, Dg, Xe, Bv, Pg纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 |
|---|---|
| Dot Blot | 1:1000 |
| WB | 1:1000 |
| IP | 1:50 |
Proto-oncogene tyrosine-protein kinase Src, also known as proto-oncogene c-Src, or simply c-Src belongs to a family of Src family kinases. c-Src phosphorylates specific tyrosine residues in other tyrosine kinases. It plays a role in the regulation of embryonic development and cell growth. An elevated level of activity of c-Src is suggested to be linked to cancer progression by promoting other signals. Mutations in c-Src could be involved in the malignant progression of colon cancer. The activation of c-Src causes the dephosphorylation of the tyrosine 527. This induces long-range allostery via protein domain dynamics, causing the structure to be destabilized, resulting in the opening up of the SH3, SH2 and kinase domains and the autophosphorylation of the residue tyrosine 419.
免疫印迹
WB result of Phospho-SRC (Tyr419) Recombinant Rabbit mAb
Primary antibody: Phospho-SRC (Tyr419) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated A431 whole cell lysate 20 µg
Lane 2: A431 treated with 50 mM pervanadate for 5 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 60 kDa
Observed MW: 60 kDaWB result of Phospho-SRC (Tyr419) Recombinant Rabbit mAb
Primary antibody: Phospho-SRC (Tyr419) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Untreated C6 whole cell lysate 20 µg
Lane 2: C6 treated with pervanadate(10mM, 20min) whole cell lysate 20 µg
Negative control: Untreated C6 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 60 kDa
Observed MW: 60kDa
免疫沉淀
Phospho-SRC (Tyr419) Recombinan Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating Phospho-SRC (Tyr419) in 0.4 mg A431 treated with 50mM pervanadate for 5 minutes whole cell lysate.
Western blot was performed on the immunoprecipitate using Phospho-SRC (Tyr419) Recombinan Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/1000 dilution.
Lane 1: A431 treated with 50mM pervanadate for 5 minutes whole cell lysate 20 µg (Input)
Lane 2: Phospho-SRC (Tyr419) Rabbit mAb IP in A431 treated with 50mM pervanadate for 5 minutes whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in A431 treated with 50mM pervanadate for 5 minutes whole cell lysate
Predicted MW: 60 kDa
Observed MW: 60kDa
斑点杂交
Dot blot result of Phospho-SRC (Tyr419) Recombinant Rabbit mAb
Lane 1: Phospho-SRC (Tyr419) peptide
Lane 2: SRC unmodified peptide
Primary antibody: Phospho-SRC (Tyr419) Recombinant Rabbit mAb at 1/1000 dilution
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
免疫细胞化学
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