所有图片（1/9）

Galectin-1 Recombinant Rabbit mAb (S-1221-32）

Gal-1,14 kDa laminin-binding protein (HLBP14),14 kDa lectin,Beta-galactoside-binding lectin L-14-I,Galaptin,HBL,HPL,Lactose-binding lectin 1,Lectin galactoside-binding soluble 1,Putative MAPK-activating protein PM12,S-Lac lectin 1,LGALS1

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0828

规格

25μl100μl1ml

数量

产品介绍

产品介绍 FAQs 评论(0)

产品规格

宿主来源

Rabbit
抗原名称

Galectin-1
分子别名

Gal-1, 14 kDa laminin-binding protein (HLBP14), 14 kDa lectin, Beta-galactoside-binding lectin L-14-I, Galaptin, HBL, HPL, Lactose-binding lectin 1, Lectin galactoside-binding soluble 1, Putative MAPK-activating protein PM12, S-Lac lectin 1, LGALS1
免疫原

Synthetic Peptide
细胞定位

Secreted, Cytoplasm
Accession

P09382
克隆号

S-1221-32
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu, Ms, Rt
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

IHC-P ?

ICC ?

WB

IP
稀释度

应用稀释度推荐种属

WB 1:1000 Hu, Ms, Rt
IHC-P 1:1000 Hu
ICC 1:500 Hu
IP 1:50 Hu

应用	稀释度	推荐种属
WB	1:1000	Hu, Ms, Rt
IHC-P	1:1000	Hu
ICC	1:500	Hu
IP	1:50	Hu

背景介绍

Galectin-1, a member of the galectin family of proteins, is a versatile and multifunctional molecule that plays pivotal roles in various biological processes. Galectin-1 supports tumor growth and metastasis by promoting tumor angiogenesis, inhibiting T-cell-mediated cytotoxic immune responses, and other mechanisms. The expression or overexpression of Galectin-1 in tumor and/or tumor-adjacent tissues serves as a marker of malignant progression and a potential diagnostic, prognostic, and therapeutic biomarker for cancer. Galectin-1 exhibits pleiotropic binding activities, modulating diverse immune cells primarily to promote immune tolerance and downregulate innate and adaptive immune responses. Galectin-1 is upregulated during inflammation and infection, modulating immune cells under both physiological and pathological conditions. It controls immune cell homeostasis and regulates acute and chronic inflammation by inhibiting the synthesis of proinflammatory cytokines, participating in T cell apoptosis programs, promoting the expansion of regulatory T cells (Tregs), and inactivating antigen-presenting cells. Galectin-1 is also essential for a healthy pregnancy and serves as a valuable biomarker for early diagnosis of preeclampsia (PE).

免疫印迹
- WB result of Galectin-1 Recombinant Rabbit mAb
  Primary antibody: Galectin-1 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: LNCaP whole cell lysate 20 µg
  Lane 2: HeLa whole cell lysate 20 µg
  Lane 3: A549 whole cell lysate 20 µg
  Negative control: LNCaP whole cell lysate
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 15 kDa
  Observed MW: 13 kDa
- WB result of Galectin-1 Recombinant Rabbit mAb
  Primary antibody: Galectin-1 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: NIH/3T3 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 15 kDa
  Observed MW: 13 kDa
- WB result of Galectin-1 Recombinant Rabbit mAb
  Primary antibody: Galectin-1 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: PC-12 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 15 kDa
  Observed MW: 13 kDa
免疫沉淀
- Galectin-1 Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating Galectin-1 in 0.4 mg HeLa whole cell lysate.
  Western blot was performed on the immunoprecipitate using Galectin-1 Rabbit mAb at 1/1000 dilution.
  Secondary antibody (HRP) for IP was used at 1/1000 dilution.
  Lane 1: HeLa whole cell lysate 20 µg (Input)
  Lane 2: Galectin-1 Rabbit mAb IP in HeLa whole cell lysate
  Lane 3: Rabbit monoclonal IgG IP in HeLa whole cell lysate
  Predicted MW: 15 kDa
  Observed MW: 13 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human tonsil. Anti- Galectin-1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human testis. Anti- Galectin-1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human kidney. Anti- Galectin-1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human colon cancer. Anti- Galectin-1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
- ICC shows positive staining in HeLa cells (top panel) and negative staining in LNCaP cells (below panel). Anti-Galectin-1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).