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宿主来源
Rabbit抗原名称
Bim分子别名
Bcl-2-like protein 11, Bcl2-L-11, Bcl2-interacting mediator of cell death, BCL2L11免疫原
Synthetic PeptideAccession
O43521克隆号
S-994-29抗体类型
Recombinant mAb抗体同种型
IgG应用
IHC-P, WB反应种属 ?
Hu, Ms, Rt纯化方式
Protein A浓度
2 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 |
|---|---|
| WB | 1:4000 |
| IHC-P | 1:100 |
BIM belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. BIM contains a Bcl-2 homology domain 3 (BH3). It has been shown to interact with other members of the BCL-2 protein family, including BCL2, BCL2L1/BCL-X(L), and MCL1, and to act as an apoptotic activator. Bim expression and activity are regulated at the transcriptional, translational and post-translational levels; coordinated expression and activity of Bim shape immune responses, and ensure tissue integrity. Cancer cells develop mechanisms that suppress Bim expression, which allows for tumor progression and metastasis.
免疫印迹
WB result of Bim Recombinant Rabbit mAb
Primary antibody: Bim Recombinant Rabbit mAb at 1/4000 dilution
Lane 1: Raji whole cell lysate 20 µg
Lane 2: Jurkat whole cell lysate 20 µg
Lane 3: A431 whole cell lysate 20 µg
Lane 4: Molt-4 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 12, 15, 23 kDa
Observed MW: 12, 15, 23 kDaWB result of Bim Recombinant Rabbit mAb
Primary antibody: Bim Recombinant Rabbit mAb at 1/4000 dilution
Lane 1: RAW264.7 whole cell lysate 20 µg
Lane 2: mouse spleen lysate 20 µg
Lane 3: mouse thymus lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 12, 15, 23 kDa
Observed MW: 12, 15, 23 kDaWB result of Bim Recombinant Rabbit mAb
Primary antibody: Bim Recombinant Rabbit mAb at 1/4000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Lane 2: rat spleen lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 12, 15, 23 kDa
Observed MW: 12, 15, 23 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-Bim antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervical cancer. Anti-Bim antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-Bim antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung cancer. Anti-Bim antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
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