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Hes1 Recombinant Rabbit mAb (S-780-211)

Transcription factor HES-1,Class B basic helix-loop-helix protein 39 (bHLHb39),Hairy and enhancer of split 1,Hairy homolog,Hairy-like protein (hHL),BHLHB39,HL,HRY

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0821

规格

25μl100μl1ml

数量

产品介绍

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产品规格

宿主来源

Rabbit
抗原名称

Hes1
分子别名

Transcription factor HES-1, Class B basic helix-loop-helix protein 39 (bHLHb39), Hairy and enhancer of split 1, Hairy homolog, Hairy-like protein (hHL), BHLHB39, HL, HRY
免疫原

Synthetic Peptide
细胞定位

Nucleus
Accession

Q14469
克隆号

S-780-211
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu, Ms, Rt
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

WB

IP
稀释度

应用稀释度推荐种属

WB 1:1000 Hu, Ms, Rt
IP 1:50 Hu

应用	稀释度	推荐种属
WB	1:1000	Hu, Ms, Rt
IP	1:50	Hu

背景介绍

Hes1 belongs to the basic helix-loop-helix (bHLH) family of transcription factors. It is a transcriptional repressor of genes that require a bHLH protein for their transcription. The protein has a particular type of basic domain that contains a helix interrupting protein that binds to the N-box promoter region rather than the canonical enhancer box (E-box). As a member of the bHLH family, it is a transcriptional repressor that influences cell proliferation and differentiation in embryogenesis. HES1 regulates its own expression via a negative feedback loop, and oscillates with approximately 2-hour periodicity. HES1 influences the maintenance of certain stem cells and progenitor cells. Specifically, HES1 influences the timing of differentiation by repressing bHLH activators, and determines binary cell fate. HES1 has been shown to play a large role in both the nervous, and digestive systems. HES1 has been shown to influence these two systems partially through the Notch signaling pathway.

免疫印迹
- WB result of Hes1 Recombinant Rabbit mAb
  Primary antibody: Hes1 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: Molt-4 whole cell lysate 20 µg
  Lane 2: MCF-7 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 30 kDa
  Observed MW: 35 kDa
- WB result of Hes1 Recombinant Rabbit mAb
  Primary antibody: Hes1 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: mouse brain lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 30 kDa
  Observed MW: 35 kDa
- WB result of Hes1 Recombinant Rabbit mAb
  Primary antibody: Hes1 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: rat testis lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 30 kDa
  Observed MW: 37 kDa
免疫沉淀
- Hes1 Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating Hes1 in 0.4 mg Molt-4 whole cell lysate.
  Western blot was performed on the immunoprecipitate using Hes1 Rabbit mAb at 1/1000 dilution.
  Secondary antibody (HRP) for IP was used at 1/1000 dilution.
  Lane 1: Molt-4 whole cell lysate 20 µg (Input)
  Lane 2: Hes1 Rabbit mAb IP in Molt-4 whole cell lysate
  Lane 3: Rabbit monoclonal IgG IP in Molt-4 whole cell lysate
  Predicted MW: 30 kDa
  Observed MW: 35 kDa