热搜词：

CN

  • CN
  • EN

注册

产品中心 抗体 其他一抗

所有图片（1/4）

Hes1 Recombinant Rabbit mAb (S-780-211)

Transcription factor HES-1,Class B basic helix-loop-helix protein 39 (bHLHb39),Hairy and enhancer of split 1,Hairy homolog,Hairy-like protein (hHL),BHLHB39,HL,HRY

货号： S0B0821

Datasheet COA
  • 价格： ￥600
  • 规格：
  • 数量：
申请试用 斯达特大包装询价 大包装询价

产品介绍 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    Hes1

  • 分子别名

    Transcription factor HES-1, Class B basic helix-loop-helix protein 39 (bHLHb39), Hairy and enhancer of split 1, Hairy homolog, Hairy-like protein (hHL), BHLHB39, HL, HRY

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Nucleus

  • Accession

    Q14469

  • 克隆号

    S-780-211

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 应用

    WB, IP

  • 反应种属 ?

    Hu, Ms, Rt

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度
应用 稀释度
WB 1:1000
IP 1:50
背景介绍

  • Hes1 belongs to the basic helix-loop-helix (bHLH) family of transcription factors. It is a transcriptional repressor of genes that require a bHLH protein for their transcription. The protein has a particular type of basic domain that contains a helix interrupting protein that binds to the N-box promoter region rather than the canonical enhancer box (E-box). As a member of the bHLH family, it is a transcriptional repressor that influences cell proliferation and differentiation in embryogenesis. HES1 regulates its own expression via a negative feedback loop, and oscillates with approximately 2-hour periodicity. HES1 influences the maintenance of certain stem cells and progenitor cells. Specifically, HES1 influences the timing of differentiation by repressing bHLH activators, and determines binary cell fate. HES1 has been shown to play a large role in both the nervous, and digestive systems. HES1 has been shown to influence these two systems partially through the Notch signaling pathway.

  • 免疫印迹

    • WB result of Hes1 Recombinant Rabbit mAb
      Primary antibody: Hes1 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: Molt-4 whole cell lysate 20 µg
      Lane 2: MCF-7 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 30 kDa
      Observed MW: 35 kDa

    • WB result of Hes1 Recombinant Rabbit mAb
      Primary antibody: Hes1 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: mouse brain lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 30 kDa
      Observed MW: 35 kDa

    • WB result of Hes1 Recombinant Rabbit mAb
      Primary antibody: Hes1 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: rat testis lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 30 kDa
      Observed MW: 37 kDa

  • 免疫沉淀

    • Hes1 Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating Hes1 in 0.4 mg Molt-4 whole cell lysate.
      Western blot was performed on the immunoprecipitate using Hes1 Rabbit mAb at 1/1000 dilution.
      Secondary antibody (HRP) for IP was used at 1/1000 dilution.
      Lane 1: Molt-4 whole cell lysate 20 µg (Input)
      Lane 2: Hes1 Rabbit mAb IP in Molt-4 whole cell lysate
      Lane 3: Rabbit monoclonal IgG IP in Molt-4 whole cell lysate
      Predicted MW: 30 kDa
      Observed MW: 35 kDa

    评论(0)