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宿主来源
Mouse抗原名称
CD235a/b分子别名
Glycophorin-A, MN sialoglycoprotein, PAS-2, Sialoglycoprotein alpha, GYPA, Glycophorin-B, PAS-3, SS-active sialoglycoprotein, Sialoglycoprotein delta, GYPB细胞定位
Cell membraneAccession
P02724, P06028克隆号
HIR2抗体类型
Recombinant mAb抗体同种型
IgG2b,k反应种属 ?
Hu纯化方式
Protein A浓度
2 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied.应用
FCM
稀释度
应用 稀释度 FCM 1:200
Glycophorin A (GYPA) and B (GYPB; this protein) are major sialoglycoproteins of the human erythrocyte membrane which bear the antigenic determinants for the MN and Ss blood groups respectively. In addition to the M or N and S or s antigens, that commonly occur in all populations, about 40 related variant phenotypes have been identified. These variants include the Miltenberger (Mi) complex and several isoforms of Stones (Sta); also Dantu, Sat, Henshaw (He or MNS6), Mg and deletion variants Ena, S-s-U- and Mk. Most of these are the result of gene recombinations between GYPA and GYPB.
流式分析
Flow cytometric analysis of MOLT-4 (Human embryonic kidney epithelial cell Human lymphoblastic leukemia T lymphoblast, left) / K562 (Human chronic myelogenous leukemia lymphoblast, right) labelling CD235a/b antibody at 1/200 dilution (1 μg) / (Red) compared with a Mouse monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Mouse IgG Alexa Fluor® 488 was used as the secondary antibody.
Negative control: MOLT-4
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