所有图片（1/9）

ACSL1 Recombinant Rabbit mAb (S-811-150)

Long-chain-fatty-acid--CoA ligase 1,Acyl-CoA synthetase 1 (ACS1),Arachidonate--CoA ligase,Long-chain acyl-CoA synthetase 1 (LACS 1),Long-chain acyl-CoA synthetase 2 (LACS 2),Long-chain fatty acid-CoA ligase 2,Palmitoyl-CoA ligase 1,Palmitoyl-CoA ligase 2,Phytanate--CoA ligase

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0817

规格

25μl100μl1ml

数量

产品介绍 FAQs 评论(0)

产品规格

宿主来源

Rabbit
抗原名称

ACSL1
分子别名

Long-chain-fatty-acid--CoA ligase 1; Acyl-CoA synthetase 1 (ACS1); Arachidonate--CoA ligase; Long-chain acyl-CoA synthetase 1 (LACS 1); Long-chain acyl-CoA synthetase 2 (LACS 2); Long-chain fatty acid-CoA ligase 2; Palmitoyl-CoA ligase 1; Palmitoyl-CoA ligase 2; Phytanate--CoA ligase
免疫原

Synthetic Peptide
Accession

P33121
克隆号

S-811-150
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu, Ms, Rt
预测反应种属
(反应种属缩写表)

GP
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

ICFCM ,
IHC-P ? ,
ICC ? ,
WB ,
IP
稀释度

应用稀释度推荐种属

WB 1:1000 Hu
IP 1:50 Hu
IHC-P 1:500-1:2000 Hu, Ms, Rt
ICC 1:500 Hu
ICFCM 1:50 Hu

应用	稀释度	推荐种属
WB	1:1000	Hu
IP	1:50	Hu
IHC-P	1:500-1:2000	Hu, Ms, Rt
ICC	1:500	Hu
ICFCM	1:50	Hu

背景介绍

ACSL1 is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. ACSL1 is known to be involved in fatty-acid metabolism critical for heart function [10] and nonspecific mental retardation. Since the ACSL4 gene is highly expressed in brain, where it encodes a brain specific isoform, an ASCL1 mutation may be an efficient diagnostic tool in mentally retarded males.

免疫印迹
- WB result of ACSL1 Recombinant Rabbit mAb
  Primary antibody: ACSL1 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: MCF-7 whole cell lysate 20 µg
  Lane 2: HepG2 whole cell lysate 20 µg
  Lane 3: HeLa whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 78 kDa
  Observed MW: 70 kDa
流式分析
- Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized HepG2 (Human hepatocellular carcinoma epithelial cell) labelling ACSL1 antibody at 1/50 dilution (1 μg) / (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫沉淀
- ACSL1 Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating ACSL1 in 0.4 mg HepG2 whole cell lysate.
  Western blot was performed on the immunoprecipitate using ACSL1 Rabbit mAb at 1/1000 dilution.
  Secondary antibody (HRP) for IP was used at 1/1000 dilution.
  Lane 1: HepG2 whole cell lysate 20 µg (Input)
  Lane 2: ACSL1 Rabbit mAb IP in HepG2 whole cell lysate
  Lane 3: Rabbit monoclonal IgG IP in HepG2 whole cell lysate
  Predicted MW: 78 kDa
  Observed MW: 70 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human kidney. Anti-ACSL1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human liver. Anti-ACSL1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-ACSL1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse cardiac muscle. Anti-ACSL1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat cardiac muscle. Anti-ACSL1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
- ICC shows positive staining in HepG2 cells. Anti- ACSL1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).