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宿主来源
Rabbit抗原名称
IFN-γ分子别名
Interferon gamma; IFNG免疫原
Recombinant Protein细胞定位
SecretedAccession
P01579克隆号
S-212-145抗体类型
Recombinant mAb抗体同种型
IgG应用
ICFCM, ICC, WB, IP反应种属 ?
Hu纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 |
|---|---|
| WB | 1:1000-1:5000 |
| IP | 1:50 |
| ICC | 1:500 |
| ICFCM | 1:50 |
IFN-gamma (Interferon-gamma) is a dimerized soluble cytokine that is the only member of the type II class of interferons. It is the prototype proinflammatory cytokine and is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells. A primary role for IFN-γ is the activation of macrophages to increase phagocytosis, tumoricidal properties, and intracellular killing of pathogens, particularly bacteria and fungi. IFN-gamma dimers signal through a receptor complex of two IFN-gamma R1 and two IFN-gamma R2 subunits. The R1 chain is sufficient for binding, but the R2 chain is required for signaling and receptor complex formation.
免疫印迹
WB result of IFN-γ Recombinant Rabbit mAb
Primary antibody: IFN-γ Recombinant Rabbit mAb at 1/5000 dilution
Lane 1: untreated NK-92 whole cell lysate 20 µg
Lane 2: NK-92 treated with TPA (80 nM, 5 hr), Ionomycin (3 μM, 5 hr), and Brefeldin A (300 ng/mL, last 4 hr) whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 19 kDa
Observed MW: 13, 15, 19 kDa
流式分析
Flow cytometric analysis of 4% PFA fixed 0.1% Tween 20 permeabilized NK-92, treated with 80nM TPA and 3uM Ionomycin for 5h in the presence of BFA for 4h (Red) or untreated (Green), labeling IFN-γ at 1/50 dilution (1 μg) compared with a Rabbit monoclonal IgG isotype control (Black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫细胞化学
ICC analysis of NK-92 cells treated with TPA (80 nM, 5 hr), Ionomycin (3 μM, 5 hr), and Brefeldin A (300 ng/mL, last 4 hr) (top panel) and untreated NK-92 cells (below panel). Anti- IFN-γ antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
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