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EGR2 Recombinant Rabbit mAb (S-741-136)

E3 SUMO-protein ligase EGR2,E3 SUMO-protein transferase ERG2,Early growth response protein 2 (EGR-2),Zinc finger protein Krox-20,Egr-2,Krox-20,Zfp-25

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0807

规格

25μl100μl1ml

数量

产品介绍

产品介绍 FAQs 评论(0)

产品规格

宿主来源

Rabbit
抗原名称

EGR2
分子别名

E3 SUMO-protein ligase EGR2; E3 SUMO-protein transferase ERG2; Early growth response protein 2 (EGR-2); Zinc finger protein Krox-20; Egr-2; Krox-20; Zfp-25
免疫原

Synthetic Peptide
细胞定位

Nucleus
Accession

P08152
克隆号

S-741-136
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Ms, Rt
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

IHC-P ?

WB

IP

IF ?
稀释度

应用稀释度推荐种属

WB 1:1000 Ms
IP 1:50 Ms
IHC-P 1:500 Ms, Rt
IF 1:100 Ms

应用	稀释度	推荐种属
WB	1:1000	Ms
IP	1:50	Ms
IHC-P	1:500	Ms, Rt
IF	1:100	Ms

背景介绍

EGR2 (also termed Krox20) is a transcription regulatory factor, containing three zinc finger DNA-binding sites, and is highly expressed in a population of migrating neural crest cells. It is later expressed in the neural crest derived cells of the cranial ganglion. Mutations in this gene are associated with the autosomal dominant Charcot-Marie-Tooth disease, type 1D, Dejerine–Sottas disease, and Congenital Hypomyelinating Neuropathy.[18] Two studies have linked EGR2 expression to proliferation of osteoprogenitors and cell lines derived from Ewing sarcoma, which is a highly aggressive bone-associated cancer. New research suggests that EGR2 - or the lack of it - is the reason for male baldness.

免疫印迹
- WB result of EGR2 Recombinant Rabbit mAb
  Primary antibody: EGR2 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: mouse thymus lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 50 kDa
  Observed MW: 58 kDa
免疫沉淀
- EGR2 Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating EGR2 in 0.4 mg mouse thymus lysate.
  Western blot was performed on the immunoprecipitate using EGR2 Rabbit mAb at 1/1000 dilution.
  Secondary antibody (HRP) for IP was used at 1/1000 dilution.
  Lane 1: mouse thymus lysate 20 µg (Input)
  Lane 2: EGR2 Rabbit mAb IP in mouse thymus lysate
  Lane 3: Rabbit monoclonal IgG IP in 2mouse thymus lysate
  Predicted MW: 50 kDa
  Observed MW: 58 kDa
  This blot was developed with high sensitivity substrate
免疫组化
- IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-EGR2 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- Negative control: IHC shows negative staining in paraffin-embedded mouse colon. Anti-EGR2 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- Negative control: IHC shows negative staining in paraffin-embedded mouse liver. Anti-EGR2 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse spleen. Anti-EGR2 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse thymus. Anti-EGR2 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse skin. Anti-EGR2 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-EGR2 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫荧光
- IF shows positive staining in paraffin-embedded mouse brain. Anti-EGR2 antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.