Cardiac Troponin I Recombinant Rabbit mAb (S-1352-28)

TNNI3,TNNC1,cTnl

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0804

规格

25μl100μl1ml

数量

产品介绍

产品介绍 FAQs 评论(0)

产品规格

宿主来源

Rabbit
抗原名称

Cardiac Troponin I
分子别名

TNNI3, TNNC1, cTnl
免疫原

Synthetic Peptide
细胞定位

Cytosol
Accession

P19429
克隆号

S-1352-28
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

IHC-P ?

IF ?
稀释度

应用稀释度

IHC-P 1:500
IF 1:500

应用	稀释度
IHC-P	1:500
IF	1:500

背景介绍

Cardiac Troponin I is a regulatory protein specifically expressed in the heart. It is one of the three subunits that constitute the troponin complex in striated muscle, mediating cardiac relaxation by inhibiting the binding of myosin and actin during muscle contraction. cTnI exhibits high myocardial specificity, being expressed exclusively in cardiac tissue. This feature endows it with exceptional accuracy in diagnosing myocardial injury. cTnI is also highly sensitive to myocardial damage, rapidly releasing into the bloodstream upon injury, allowing for its detection.

免疫组化
- IHC shows positive staining in paraffin-embedded human cardiac muscle. Anti-Cardiac Troponin I antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- Negative control: IHC shows negative staining in paraffin-embedded human kidney. Anti-Cardiac Troponin I antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- Negative control: IHC shows negative staining in paraffin-embedded human skeletal muscle. Anti-Cardiac Troponin I antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- Negative control: IHC shows negative staining in paraffin-embedded human prostatic hyperplasia. Anti-Cardiac Troponin I antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- Negative control: IHC shows negative staining in paraffin-embedded human ovarian cancer. Anti-Cardiac Troponin I antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫荧光
- IF shows positive staining in paraffin-embedded human cardiac muscle. Anti- Cardiac Troponin I antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
- Negative control: IF shows negative staining in paraffin-embedded human cerebral cortex. Anti- Cardiac Troponin I antibody was used at 1/500 dilution and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.