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α-tubulin Recombinant Mouse mAb (HRP Conjugate) (S-364-23HL)

Tubulin alpha-4A chain,Alpha-tubulin 1

货号： S0B0800

Datasheet COA
  • 价格： ￥480
  • 规格：
  • 数量：
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产品介绍 评论(0)

产品规格

  • 宿主来源

    Mouse

  • 抗原名称

    α-tubulin

  • 分子别名

    Tubulin alpha-4A chain, Alpha-tubulin 1

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Cytoplasm, Cytoskeleton

  • Accession

    P68366

  • 克隆号

    S-364-23HL

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG1,k

  • 应用

    WB

  • 反应种属 ?

    Hu, Ms, Rt

  • 纯化方式

    Protein G

  • 浓度

    2 mg/ml

  • 标记

    HRP

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied.
稀释度
应用 稀释度
WB 1:1000-1:5000
背景介绍

  • Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Tubulin α- and β-subunits have molecular weights of ~ 50 kDa and are 36%–42% identical and 63% homologous. Both tubulin subunits bind guanine nucleotides. The binding to α-tubulin at the N-site is nonexchangeable, while the binding to β-tubulin at the E-site is exchangeable. Nucleotide in microtubules does not exchange with the solution, except for terminal subunits at microtubule ends.

  • 免疫印迹

    • WB result of α-tubulin Recombinant Mouse mAb (HRP Conjugate)
      Primary antibody: α-tubulin Recombinant Mouse mAb (HRP Conjugate) at 1/1000 dilution
      Lane 1: HeLa whole cell lysate 20 µg
      Lane 2: NIH/3T3 whole cell lysate 20 µg
      Lane 3: C6 whole cell lysate 20 µg
      Predicted MW: 52 kDa
      Observed MW: 55 kDa

    • WB result of α-tubulin Recombinant Mouse mAb (HRP Conjugate)
      Primary antibody: Pα-tubulin Recombinant Mouse mAb (HRP Conjugate) at 1/5000 dilution
      Lane 1: HeLa whole cell lysate 20 µg
      Lane 2: NIH/3T3 whole cell lysate 20 µg
      Lane 3: C6 whole cell lysate 20 µg
      Predicted MW: 52 kDa
      Observed MW: 55 kDa

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