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MDM2 Recombinant Rabbit mAb (S-1236-49)

E3 ubiquitin-protein ligase Mdm2,Double minute 2 protein (Hdm2),Oncoprotein Mdm2,RING-type E3 ubiquitin transferase Mdm2,p53-binding protein Mdm2

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0798

规格

25μl100μl1ml

数量

产品介绍

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产品规格

宿主来源

Rabbit
抗原名称

MDM2
分子别名

E3 ubiquitin-protein ligase Mdm2, Double minute 2 protein (Hdm2), Oncoprotein Mdm2, RING-type E3 ubiquitin transferase Mdm2, p53-binding protein Mdm2
免疫原

Recombinant Protein
细胞定位

Cytoplasm, Nucleus
Accession

Q00987
克隆号

S-1236-49
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu, Ms
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

ICFCM ,
ICC ? ,
WB
稀释度

应用稀释度推荐种属

WB 1:1000 Hu, Ms
ICC 1:500 Hu
ICFCM 1:500 Hu

应用	稀释度	推荐种属
WB	1:1000	Hu, Ms
ICC	1:500	Hu
ICFCM	1:500	Hu

背景介绍

As an E3 ubiquitin-protein ligase, MDM2 is involved in the ubiquitination and subsequent degradation of various proteins, primarily targeting the tumor suppressor protein p53/TP53. By mediating p53's degradation, MDM2 regulates cell cycle progression, apoptosis, and DNA damage repair. In normal cells, MDM2 maintains a delicate balance with p53, jointly modulating cell cycle arrest, apoptosis, and DNA repair to ensure cellular homeostasis. In cancer, MDM2 is frequently overexpressed, leading to increased degradation of p53, thereby inhibiting apoptosis and cell cycle arrest, contributing to tumorigenesis. MDM2 overexpression is also associated with tumor microenvironment remodeling and immune evasion. Furthermore, MDM2 exerts bidirectional effects on CD8+ T cells, CD4+ T cells, and other immune cells like NK cells, DCs, Tregs, and TAMs, shaping the tumor immune microenvironment.

免疫印迹
- WB result of MDM2 Recombinant Rabbit mAb
  Primary antibody: MDM2 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: HeLa whole cell lysate 20 µg
  Lane 2: SK-OV-3 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 55 kDa
  Observed MW: 75 kDa
- WB result of MDM2 Recombinant Rabbit mAb
  Primary antibody: MDM2 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: RAW264.7 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 55 kDa
  Observed MW: 75 kDa
流式分析
- Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) labelling MDM2 antibody at 1/500 dilution (0.1 μg)/ (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫细胞化学
- ICC analysis of U-2 OS cells treated with Nutlin 3a (10 μM, 24 hr) (top panel) and U-2 OS cells untreated with Nutlin 3a (10 μM, 24 hr) (below panel). Anti-MDM2 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).