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JNK2 Recombinant Rabbit mAb (S-1021-46)

Mitogen-activated protein kinase 9,MAP kinase 9,MAPK9,JNK-55,Stress-activated protein kinase 1a (SAPK1a),Stress-activated protein kinase JNK2,c-Jun N-terminal kinase 2,PRKM9,SAPK1A

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0797

规格

25μl100μl1ml

数量

产品介绍

产品介绍 FAQs 评论(0)

产品规格

宿主来源

Rabbit
抗原名称

JNK2
分子别名

Mitogen-activated protein kinase 9, MAP kinase 9, MAPK9, JNK-55, Stress-activated protein kinase 1a (SAPK1a), Stress-activated protein kinase JNK2, c-Jun N-terminal kinase 2, PRKM9, SAPK1A
免疫原

Synthetic Peptide
细胞定位

Cytoplasm, Nucleus
Accession

P45984
克隆号

S-1021-46
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu, Ms, Rt
预测反应种属
(反应种属缩写表)

Av
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

ICFCM

ICC ?

WB

IP
稀释度

应用稀释度推荐种属

WB 1:1000 Hu, Ms, Rt
IP 1:50 Hu
ICC 1:500 Hu, Ms
ICFCM 1:50 Hu, Ms

应用	稀释度	推荐种属
WB	1:1000	Hu, Ms, Rt
IP	1:50	Hu
ICC	1:500	Hu, Ms
ICFCM	1:50	Hu, Ms

背景介绍

Jun N-terminal Kinase 2, also known as JNK2, is a member of the mitogen-activated protein kinase (MAPK) family, specifically belonging to the subgroup of stress-activated protein kinases (SAPKs). JNK2 plays a pivotal role in the regulation of cell survival, apoptosis, proliferation, differentiation, and inflammatory responses. Upon activation by upstream signaling molecules, JNK2 phosphorylates various transcription factors, particularly members of the c-Jun family (including c-Jun itself), as well as ATF2, p53, and others. This phosphorylation event modulates the transcriptional activity of these factors, ultimately influencing the expression of genes involved in the aforementioned cellular processes. The activation of JNK2 is a complex process that involves the phosphorylation of specific threonine and tyrosine residues within its activation loop by MAPK kinases (MKKs), primarily MKK4 and MKK7. This phosphorylation event triggers a conformational change in JNK2, allowing it to become fully active and capable of phosphorylating its downstream substrates. The upstream signaling pathways that lead to JNK2 activation are diverse and include pathways activated by TNF-α, IL-1, and other inflammatory cytokines, as well as pathways activated by environmental stresses such as UV radiation and oxidative stress.

免疫印迹
- WB result of JNK2 Recombinant Rabbit mAb
  Primary antibody: JNK2 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: HeLa whole cell lysate 20 µg
  Lane 2: K562 whole cell lysate 20 µg
  Lane 3: MCF7 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 48 kDa
  Observed MW: 46, 54 kDa
- WB result of JNK2 Recombinant Rabbit mAb
  Primary antibody: JNK2 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: NIH/3T3 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 48 kDa
  Observed MW: 46, 54 kDa
- WB result of JNK2 Recombinant Rabbit mAb
  Primary antibody: JNK2 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: PC-12 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 48 kDa
  Observed MW: 46, 54 kDa
流式分析
- Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) labelling JNK2 antibody at 1/50 dilution (1 μg)/ (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
- Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized NIH/3T3 (Mouse embryonic fibroblast) labelling JNK2 antibody at 1/50 dilution (1 μg)/ (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫沉淀
- JNK2 Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating JNK2 in 0.4 mg HeLa whole cell lysate.
  Western blot was performed on the immunoprecipitate using JNK2 Rabbit mAb at 1/1000 dilution.
  Secondary antibody (HRP) for IP was used at 1/1000 dilution.
  Lane 1: HeLa whole cell lysate 20 µg (Input)
  Lane 2: JNK2 Rabbit mAb IP in HeLa whole cell lysate
  Lane 3: Rabbit monoclonal IgG IP in HeLa whole cell lysate
  Predicted MW: 48 kDa
  Observed MW: 46, 54 kDa
免疫细胞化学
- ICC shows positive staining in HeLa cells. Anti-JNK2 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
- ICC shows positive staining in NIH/3T3 cells. Anti-JNK2 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).