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IKK alpha/beta Recombinant Rabbit mAb (S-1317-27)

Inhibitor of nuclear factor kappa-B kinase subunit alpha,I-kappa-B kinase alpha,IKK-A,IkBKA,IkappaB kinase,Conserved helix-loop-helix ubiquitous kinase,I-kappa-B kinase 1,Nuclear factor NF-kappa-B inhibitor kinase alpha (NFKBIKA),Transcription factor 16 (TCF-16),CHUK,Inhibitor of nuclear factor kappa-B kinase subunit beta,I-kappa-B-kinase beta,IKK-B,IkBKB,I-kappa-B kinase 2,Nuclear factor NF-kappa-B inhibitor kinase beta (NFKBIKB),Serine/threonine protein kinase IKBKB,IKBKB

货号： S0B0796

Datasheet COA
  • 价格： ￥600
  • 规格：
  • 数量：
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产品介绍 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    IKK alpha/beta

  • 分子别名

    Inhibitor of nuclear factor kappa-B kinase subunit alpha, I-kappa-B kinase alpha, IKK-A, IkBKA, IkappaB kinase, Conserved helix-loop-helix ubiquitous kinase, I-kappa-B kinase 1, Nuclear factor NF-kappa-B inhibitor kinase alpha (NFKBIKA), Transcription factor 16 (TCF-16), CHUK, Inhibitor of nuclear factor kappa-B kinase subunit beta, I-kappa-B-kinase beta, IKK-B, IkBKB, I-kappa-B kinase 2, Nuclear factor NF-kappa-B inhibitor kinase beta (NFKBIKB), Serine/threonine protein kinase IKBKB, IKBKB

  • 免疫原

    Recombinant Protein

  • 细胞定位

    Cytoplasm, Nucleus

  • Accession

    O14920, O15111

  • 克隆号

    S-1317-27

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 应用

    ICFCM, ICC, WB, IP

  • 反应种属 ?

    Hu, Ms, Rt

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度
应用 稀释度
WB 1:1000
IP 1:50
ICC 1:50
ICFCM 1:50
背景介绍

  • The Inhibitor of Nuclear Factor Kappa-B Kinase (IKK) is a pivotal serine/threonine kinase complex that plays a crucial role in regulating the Nuclear Factor Kappa-B (NF-κB) signaling pathway. This pathway is fundamental for controlling diverse physiological and pathological processes, including immune responses, inflammation, cell proliferation, and apoptosis. The IKK complex comprises three main subunits: IKKα (also known as IKK1), IKKβ (IKK2), and IKKγ (or NEMO/IKBKG). Each subunit contributes uniquely to the complex's structure and function. IKKα possesses kinase activity, albeit lower than IKKβ. Its precise role in NF-κB activation remains somewhat elusive, but it may be involved in specific types of NF-κB activation. IKKβ serves as the primary kinase subunit within the complex, exhibiting robust kinase activity. IKKβ is essential for activating the NF-κB pathway by phosphorylating the inhibitory protein IκB, leading to its degradation and subsequent release of NF-κB for nuclear translocation and transcriptional activation. IKK's primary function lies in the activation of the NF-κB signaling pathway. By phosphorylating specific serine residues on the IκB proteins, IKK triggers their ubiquitination and subsequent degradation by the proteasome. This process frees NF-κB from inhibition, allowing it to translocate to the nucleus and activate the transcription of target genes.

  • 免疫印迹

    • WB result of IKK alpha/beta Recombinant Rabbit mAb
      Primary antibody: IKK alpha/beta Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: HeLa whole cell lysate 20 µg
      Lane 2: HepG2 whole cell lysate 20 µg
      Lane 3: HCT 116 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 87 kDa
      Observed MW: 85, 90 kDa

    • WB result of IKK alpha/beta Recombinant Rabbit mAb
      Primary antibody: IKK alpha/beta Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: NIH/3T3 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 87 kDa
      Observed MW: 85, 90 kDa

    • WB result of IKK alpha/beta Recombinant Rabbit mAb
      Primary antibody: IKK alpha/beta Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: C6 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 87 kDa
      Observed MW: 85, 90 kDa

  • 流式分析

    • Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) labelling IKK alpha/beta antibody at 1/50 dilution (1 μg)/ (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.

  • 免疫沉淀

    • IKK alpha/beta Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating IKK alpha/beta in 0.4 mg HeLa whole cell lysate.
      Western blot was performed on the immunoprecipitate using IKK alpha/beta Rabbit mAb at 1/1000 dilution.
      Secondary antibody (HRP) for IP was used at 1/1000 dilution.
      Lane 1: HeLa whole cell lysate 20 µg (Input)
      Lane 2: IKK alpha/beta Rabbit mAb IP in HeLa whole cell lysate
      Lane 3: Rabbit monoclonal IgG IP in HeLa whole cell lysate
      Predicted MW: 87 kDa
      Observed MW: 85, 90 kDa

  • 免疫细胞化学

    • ICC shows positive staining in HeLa cells. Anti- IKK alpha beta antibody was used at 1/50 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).

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