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Rabbit抗原名称
TIMP1分子别名
Metalloproteinase inhibitor 1, Erythroid-potentiating activity (EPA), Fibroblast collagenase inhibitor (Collagenase inhibitor), Tissue inhibitor of metalloproteinases 1 (TIMP-1), CLGI, TIMP免疫原
Recombinant Protein细胞定位
SecretedAccession
P01033克隆号
S-213-124抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
WB
稀释度
应用 稀释度 WB 1:1000
TIMP-1 (Tissue Inhibitor of Metalloproteinases-1) is a crucial protein involved in the metabolism of the extracellular matrix (ECM). TIMP-1 belongs to a family of proteinases containing Zn2+ and Ca2+ ions, which are essential for their catalytic activities. TIMP-1 acts as a potent inhibitor of multiple matrix metalloproteinases (MMPs) by forming complexes with their catalytic zinc ions, thereby irreversibly inactivating them. Its inhibitory spectrum is broad, encompassing MMPs such as MMP1, MMP2, MMP3, MMP7, MMP8, MMP9, MMP10, MMP11, MMP12, MMP13, and MMP16. Beyond its enzymatic inhibitory role, TIMP-1 also engages in cell signaling by interacting with molecules like CD63 and ITGB1, modulating specific cellular signaling pathways that govern cell differentiation, migration, and apoptosis.
免疫印迹
WB result of TIMP1 Recombinant Rabbit mAb
Primary antibody: TIMP1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Ramos whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: HT-29 whole cell lysate 20 µg
Negative control: Ramos whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 23 kDa
Observed MW: 28 kDa
This blot was developed with high sensitivity substrate
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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