PKC eta Recombinant Rabbit mAb (S-1053-35)

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货号： S0B0790

Datasheet COA

价格： ￥260
规格：
10μl25μl100μl1ml
数量：

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产品规格

宿主来源

Rabbit
抗原名称

PKC eta
分子别名

Protein kinase C eta type, PKC-L, nPKC-eta, Prkch, Pkch
免疫原

Synthetic Peptide
细胞定位

Cytoplasm
Accession

P23298
克隆号

S-1053-35
抗体类型

Recombinant mAb
抗体同种型

IgG
应用

WB, IP
反应种属 ?

Hu, Ms, Rt
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度

应用	稀释度
WB	1:1000
IP	1:50

背景介绍

PKC eta, also known as Protein Kinase C eta, is a member of the Protein Kinase C (PKC) family of enzymes. This serine/threonine-specific protein kinase plays crucial roles in various cellular processes, particularly in signal transduction, cell differentiation, and gene transcription regulation. PKC eta regulates keratinocyte differentiation by activating the MAPK13 (p38delta) activated protein kinase cascade. PKC eta mediates transcriptional activation of the transglutaminase 1 (TGM1) gene. Mutations in the gene have been linked to susceptibility to cerebral infarction.

免疫印迹
- WB result of PKC eta Recombinant Rabbit mAb
  Primary antibody: PKC eta Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: MCF7 whole cell lysate 20 µg
  Lane 2: PC-3 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 78 kDa
  Observed MW: 45, 78 kDa
  This blot was developed with high sensitivity substrate
- WB result of PKC eta Recombinant Rabbit mAb
  Primary antibody: PKC eta Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: mouse lung lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 78 kDa
  Observed MW: 45, 78 kDa
  This blot was developed with high sensitivity substrate
- WB result of PKC eta Recombinant Rabbit mAb
  Primary antibody: PKC eta Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: rat lung lysate 20 µg
  Lane 2: rat skin lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 78 kDa
  Observed MW: 45, 78 kDa
  This blot was developed with high sensitivity substrate
免疫沉淀
- PKC eta Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating PKC eta in 0.4 mg mouse lung lysate.
  Western blot was performed on the immunoprecipitate using PKC eta Rabbit mAb at 1/1000 dilution.
  Secondary antibody (HRP) for IP was used at 1/1000 dilution.
  Lane 1: mouse lung lysate 20 µg (Input)
  Lane 2: PKC eta Rabbit mAb IP in mouse lung lysate
  Lane 3: Rabbit monoclonal IgG IP in mouse lung lysate
  Predicted MW: 78 kDa
  Observed MW: 45, 78 kDa