PKM2 Rabbit Polyclonal antibody
Pyruvate kinase PKM,Cytosolic thyroid hormone-binding protein (CTHBP),Opa-interacting protein 3 (OIP-3),Pyruvate kinase 2/3,Pyruvate kinase muscle isozyme,Threonine-protein kinase PKM2,Thyroid hormone-binding protein 1 (THBP1),Tumor M2-PK,Tyrosine-protein kinase PKM2,p58,PKM,OIP3,PK2,PK3
货号： S0B0785
- 价格： ￥400
- 规格：
- 数量：
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宿主来源
Rabbit抗原名称
PKM2分子别名
Pyruvate kinase PKM, Cytosolic thyroid hormone-binding protein (CTHBP), Opa-interacting protein 3 (OIP-3), Pyruvate kinase 2/3, Pyruvate kinase muscle isozyme, Threonine-protein kinase PKM2, Thyroid hormone-binding protein 1 (THBP1), Tumor M2-PK, Tyrosine-protein kinase PKM2, p58, PKM, OIP3, PK2, PK3免疫原
Synthetic Peptide细胞定位
Nucleus, CytoplasmAccession
P14618抗体类型
Polyclonal antibody抗体同种型
IgG应用
ICFCM, IHC-P, ICC, WB反应种属 ?
Hu, Ms, Rt预测反应种属
(反应种属缩写表)Or, Ct, Rb纯化方式
Immunogen Affinity浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 |
|---|---|
| WB | 1:1000 |
| IHC-P | 1:250 |
| ICC | 1:500 |
| ICFCM | 1:50 |
PKM2, also known as Pyruvate Kinase Muscle Isozyme 2, is an isoform of pyruvate kinase (PK) that is primarily found in muscle and cardiac tissues. As a key enzyme in glycolysis, PKM2 catalyzes the conversion of phosphoenolpyruvate (PEP) into pyruvate, releasing one molecule of ATP in the process. PKM2 is overexpressed in various types of tumors and has been linked to malignant biological behaviors such as tumor proliferation, invasion, and metastasis. Therefore, PKM2 is considered a potential tumor marker and therapeutic target. In addition, PKM2 plays a significant role in cellular metabolism. Under conditions of hypoxia or increased energy demand, the activity of PKM2 increases, promoting glycolysis to provide energy for cells. However, in tumor cells, the abnormally elevated activity of PKM2 may lead to excessive dependence on glucose and metabolic reprogramming, supporting tumor growth and dissemination.
免疫印迹
WB result of PKM2 Rabbit pAb
Primary antibody: PKM2 Rabbit pAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: A549 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 58 kDa
Observed MW: 60 kDaWB result of PKM2 Rabbit pAb
Primary antibody: PKM2 Rabbit pAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 58 kDa
Observed MW: 60 kDaWB result of PKM2 Rabbit pAb
Primary antibody: PKM2 Rabbit pAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 58 kDa
Observed MW: 60 kDa
流式分析
Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) labelling PKM2 antibody at 1/50 dilution (1 μg)/ (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized NIH/3T3 (Mouse embryonic fibroblast) labelling PKM2 antibody at 1/50 dilution (1 μg)/ (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫组化
IHC shows positive staining in paraffin-embedded human colon. Anti-PKM2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human testis. Anti-PKM2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-PKM2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-PKM2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse colon. Anti-PKM2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat stomach. Anti-PKM2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in HeLa cells. Anti-PKM2 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ICC shows positive staining in NIH/3T3 cells. Anti-PKM2 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
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