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Phospho-p70 S6 Kinase (Ser371) Recombinant Rabbit mAb (S-1239-6)

Ribosomal protein S6 kinase beta-1,S6K-beta-1,S6K1,70 kDa ribosomal protein S6 kinase 1 (P70S6K1,p70-S6K 1),Ribosomal protein S6 kinase I,Serine/threonine-protein kinase 14A,p70 ribosomal S6 kinase alpha (p70 S6 kinase alpha,p70 S6K-alpha,p70 S6KA),RPS6KB1,STK14A

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0784

规格

25μl100μl1ml

数量

产品介绍

产品介绍 FAQs 评论(0)

产品规格

宿主来源

Rabbit
抗原名称

Phospho-p70 S6 Kinase (Ser371)
分子别名

Ribosomal protein S6 kinase beta-1, S6K-beta-1, S6K1, 70 kDa ribosomal protein S6 kinase 1 (P70S6K1; p70-S6K 1), Ribosomal protein S6 kinase I, Serine/threonine-protein kinase 14A, p70 ribosomal S6 kinase alpha (p70 S6 kinase alpha; p70 S6K-alpha; p70 S6KA), RPS6KB1, STK14A
免疫原

Synthetic Peptide
细胞定位

Cytoplasm
Accession

P23443
克隆号

S-1239-6
抗体类型

Recombinant mAb
抗体同种型

IgG
翻译后修饰类型

磷酸化
反应种属 ?

Hu
预测反应种属
(反应种属缩写表)

Bv, Rb, Rt, Ms
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

WB

IP
稀释度

应用稀释度

Dot Blot 1:1000
WB 1:1000
IP 1:50

应用	稀释度
Dot Blot	1:1000
WB	1:1000
IP	1:50

背景介绍

Phospho-p70 S6 Kinase (Ser371) refers to the specifically activated form of p70 ribosomal protein S6 kinase (p70S6K, a key serine/threonine kinase) phosphorylated at its serine 371 residue (Ser371). As a central downstream effector of the mTORC1 (mechanistic target of rapamycin complex 1) signaling pathway, phosphorylation at this site is crucial for the full activation of p70S6K. Ser371 is located within the activation loop (T-loop) of the kinase domain. Its phosphorylation, typically catalyzed by mTORC1 itself or other upstream kinases, significantly enhances the kinase activity of p70S6K. The activated Phospho-p70 S6 Kinase (Ser371) then phosphorylates its downstream targets, such as ribosomal protein S6 and eIF4B, thereby directly promoting protein translation, ribosome biogenesis, and cell growth. Consequently, detecting the phosphorylation level at Ser371 is a commonly used biomarker for assessing mTORC1 pathway activity and studying cell proliferation, metabolic regulation, as well as drug target responses in related diseases such as cancer and metabolic disorders.

免疫印迹
- WB result of Phospho-p70 S6 Kinase (Ser371) Recombinant Rabbit mAb
  Primary antibody: Phospho-p70 S6 Kinase (Ser371) Recombinant mAb at 1/1000 dilution
  Lane 1: 293T whole cell lysate 20 µg
  Lane 2: phosphatase treated 293T whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 59 kDa
  Observed MW: 55~70 kDa
免疫沉淀
- Phospho-p70 S6 Kinase (Ser371) Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating Phospho-p70 S6 Kinase (Ser371) in 0.4 mg 293T whole cell lysate.
  Western blot was performed on the immunoprecipitate using Phospho-p70 S6 Kinase (Ser371) Rabbit mAb at 1/1000 dilution.
  Secondary antibody (HRP) for IP was used at 1/1000 dilution.
  Lane 1: 293T whole cell lysate 20 µg (Input)
  Lane 2: Phospho-p70 S6 Kinase (Ser371) Rabbit mAb IP in 293T whole cell lysate
  Lane 3: Rabbit monoclonal IgG IP in 293T whole cell lysate
  Predicted MW: 59 kDa
  Observed MW: 55~70 kDa
斑点杂交
- Dot blot result of Phospho-p70 S6 Kinase (Ser371) Recombinant mAb
  Lane 1: Phospho-p70 S6 Kinase (Ser371) peptide
  Lane 2: Phospho-p70 S6 Kinase unmodified peptide
  Primary antibody: Phospho-p70 S6 Kinase (Ser371) Recombinant mAb at 1/1000 dilution
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution