热搜词：
注册
登录
CN
  • CN
  • EN

产品中心 抗体 其他一抗

所有图片（1/3）

Phospho-Akt (Ser473) Mouse mAb (S-769-59)

RAC-alpha serine/threonine-protein kinase,Protein kinase B (PKB),Protein kinase B alpha (PKB alpha),Proto-oncogene c-Akt,RAC-PK-alpha,PKB,RAC,AKT1

价格 ￥600.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B0783
规格
数量
收藏 分享
产品介绍

产品介绍 评论(0)

产品规格

  • 宿主来源

    Mouse

  • 抗原名称

    Phospho-Akt (Ser473)

  • 分子别名

    RAC-alpha serine/threonine-protein kinase, Protein kinase B (PKB), Protein kinase B alpha (PKB alpha), Proto-oncogene c-Akt, RAC-PK-alpha, PKB, RAC, AKT1

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Cytoplasm, Nucleus, Cell membrane

  • Accession

    P31749

  • 克隆号

    S-769-59

  • 抗体类型

    Mouse mAb

  • 抗体同种型

    IgG1,k

  • 翻译后修饰类型

    磷酸化

  • 反应种属 ?

    Hu, Rt

  • 预测反应种属
    (反应种属缩写表)

    Ms

  • 纯化方式

    Protein G

  • 浓度

    2 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

  • 应用

    WB

  • 稀释度

    应用 稀释度
    Dot Blot 1:1000
    WB 1:1000
背景介绍

  • Akt or Protein kinase B, is a serine/threonine kinase that plays an important role in regulating a number of cellular processes such as growth, metabolism and survival. The importance of the Akt pathway is highlighted by the mutation of various components of the pathway in human cancers such as the PTEN and PI3-kinase (P110α), which occur in more than 30% of human tumors. For Akt to achieve full activation, phosphorylation is needed at both serine 473 (ser473) of the hydrophobic tail and threonine 308 (thr308) of the activation motif, upon growth factor ligation to the receptor tyrosine kinases.

  • 免疫印迹

    • WB result of Phospho-Akt (Ser473) Mouse mAb
      Primary antibody: Phospho-Akt (Ser473) Mouse mAb at 1/1000 dilution
      Lane 1: untreated Jurkat whole cell lysate 20 µg
      Lane 2: Jurkat starve overnight, then treated with 100 nM Calyculin A for 30 minutes whole cell lysate 20 µg
      Secondary antibody: Goat Anti-mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 56 kDa
      Observed MW: 60 kDa

    • WB result of Phospho-Akt (Ser473) Mouse mAb
      Primary antibody: Phospho-Akt (Ser473) Mouse mAb at 1/1000 dilution
      Lane 1: untreated C6 whole cell lysate 20 µg
      Lane 2: C6 treated with 100 nM Calyculin A for 30 minutes whole cell lysate 20 µg
      Secondary antibody: Goat Anti-mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 56 kDa
      Observed MW: 60 kDa

  • 斑点杂交

    • Dot blot result of Phospho-Akt (Ser473) Mouse mAb
      Lane 1: Phospho-Akt (Ser473) peptide
      Lane 2: Akt unmodified peptide
      Primary antibody: Phospho-Akt (Ser473) Mouse mAb at 1/1000 dilution
      Secondary antibody: Goat Anti-mouse IgG, (H+L), HRP conjugated at 1/10000 dilution

评论(0)