c-Rel Recombinant Rabbit mAb (S-1150-52)

Proto-oncogene c-Rel

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0779

规格

25μl100μl1ml

数量

产品介绍

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产品规格

宿主来源

Rabbit
抗原名称

c-Rel
分子别名

Proto-oncogene c-Rel
免疫原

Synthetic Peptide
细胞定位

Cytoplasm, Membrane
Accession

P15307
克隆号

S-1150-52
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Ms, Rt
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

ChIP

ICFCM

IHC-P ?

ICC ?

WB

IP
稀释度

应用稀释度

WB 1:1000
IHC-P 1:200
ICC 1:50
ICFCM 1:50
ChIP 1:20-1:50

应用	稀释度
WB	1:1000
IHC-P	1:200
ICC	1:50
ICFCM	1:50
ChIP	1:20-1:50

背景介绍

c-Rel is a member of the NF-κB family, predominantly expressed in lymphocytes and myeloid cells. As a transcription factor, it regulates the proinflammatory polarization of myeloid cells and modulates the antitumor immune response. Research has found that c-Rel serves as an important checkpoint for the immunosuppressive function of myeloid-derived suppressor cells (MDSCs). MDSCs hinder the normal function of immune cells in a tumor environment, promoting tumor immune evasion. c-Rel controls the polarization of myeloid cells in tumors, and specific inhibition of c-Rel significantly inhibits tumor growth. In c-Rel-deficient (Rel–/–) mice, melanoma and lymphoma tumor growth was significantly suppressed, with a 80% reduction in tumor size and body weight compared to controls. Inhibiting c-Rel through small molecule inhibitors or conditional knockout in MDSCs can promote the body's antitumor immune response. Combining c-Rel inhibitors with PD-1 functional blocking antibodies can further enhance the activation of the body's antitumor immune response.

免疫印迹
- WB result of c-Rel Recombinant Rabbit mAb
  Primary antibody: c-Rel Recombinant mAb at 1/1000 dilution
  Lane 1: RAW264.7 whole cell lysate 20 µg
  Lane 2: Neuro-2a whole cell lysate 20 µg
  Lane 3: mouse spleen lysate 20 µg
  Lane 4: mouse thymus lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 65 kDa
  Observed MW: 70 kDa
流式分析
- Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized Neuro-2a (Mouse neuroblastoma neuroblast) labelling c-Rel antibody at 1/50 dilution (1 μg)/ (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫组化
- IHC shows positive staining in paraffin-embedded mouse spleen. Anti- c-Rel antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse testis. Anti- c-Rel antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat spleen. Anti- c-Rel antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
- ICC shows positive staining in Neuro-2a cells. Anti- c-Rel antibody was used at 1/50 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ChIP
- Chromatin immunoprecipitation (ChIP) was performed on HeLa cells cross - linked with 1% formaldehyde for 10 min, then chromatin was fragmented by sonication. Parallel reactions used c-Rel Recombinant Rabbit mAb (S-1150-52) and Rabbit mAb IgG Isotype Control (SDT-R173) at 1:50 for immunoprecipitation.
  Post - immunoprecipitation, both samples were washed, eluted, and cross - links reversed. Purified DNA was analyzed by qPCR.
  qPCR showed the enrichment of BCL2L1, EP300 and SAT-α in c-Rel Recombinant
  Rabbit mAb (S-1150-52)-immunoprecipitated sample.