QPRT Recombinant Rabbit mAb (S-1228-31)

Nicotinate-nucleotide pyrophosphorylase [carboxylating],Quinolinate phosphoribosyltransferase [decarboxylating]

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0773

规格

25μl100μl1ml

数量

产品介绍

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产品规格

宿主来源

Rabbit
抗原名称

QPRT
分子别名

Nicotinate-nucleotide pyrophosphorylase [carboxylating], Quinolinate phosphoribosyltransferase [decarboxylating]
免疫原

Synthetic Peptide
细胞定位

Cytoplasm
Accession

Q15274
克隆号

S-1228-31
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

IHC-P ?

WB

IP
稀释度

应用稀释度

WB 1:1000
IHC-P 1:1000
IP 1:50

应用	稀释度
WB	1:1000
IHC-P	1:1000
IP	1:50

背景介绍

QPRT, also known as Quinolinate Phosphoribosyltransferase, is an enzyme that catalyzes the conversion of quinolinic acid to nicotinic acid mononucleotide (NAMN) in the biosynthetic pathway of nicotinamide adenine dinucleotide (NAD+). This reaction involves the attachment of phosphoribosyl pyrophosphate (PRPP) to quinolinic acid, forming NAMN and pyrophosphate. QPRT plays a crucial role in maintaining the cellular NAD+ pool, which is essential for cellular metabolism, DNA repair, and other cellular functions. Abnormalities in QPRT activity or expression have been linked to various diseases, including cancer, neurodegenerative disorders, and metabolic diseases. In cancer, QPRT is often overexpressed, and its activity may contribute to tumor growth and progression. This is because NAD+ is a cofactor for many enzymes involved in cellular metabolism, including those that regulate glycolysis, fatty acid synthesis, and oxidative phosphorylation. By maintaining a high level of NAD+, QPRT may support the metabolic demands of rapidly proliferating tumor cells.

免疫印迹
- WB result of QPRT Recombinant Rabbit mAb
  Primary antibody: QPRT Recombinant mAb at 1/1000 dilution
  Lane 1: MDA-MB-231 whole cell lysate 20 µg
  Lane 2: Jurkat whole cell lysate 20 µg
  Lane 3: HeLa whole cell lysate 20 µg
  Lane 4: MCF7 whole cell lysate 20 µg
  Negative control: MDA-MB-231 whole cell lysate
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 31 kDa
  Observed MW: 37 kDa
免疫沉淀
- QPRT Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating QPRT in 0.4 mg HepG2 whole cell lysate.
  Western blot was performed on the immunoprecipitate using QPRT Rabbit mAb at 1/1000 dilution.
  Secondary antibody (HRP) for IP was used at 1/1000 dilution.
  Lane 1: HepG2 whole cell lysate 20 µg (Input)
  Lane 2: QPRT Rabbit mAb IP in HepG2 whole cell lysate
  Lane 3: Rabbit monoclonal IgG IP in HepG2 whole cell lysate
  Predicted MW: 31 kDa
  Observed MW: 37 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human kidney. Anti- QPRT antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human liver. Anti- QPRT antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human testis. Anti- QPRT antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human tonsil. Anti- QPRT antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human prostatic hyperplasia. Anti- QPRT antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human colon cancer. Anti- QPRT antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human lung adenocarcinoma. Anti- QPRT antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.