VPS35 Recombinant Rabbit mAb (S-997-2)

Vacuolar protein sorting-associated protein 35,hVPS35,Maternal-embryonic 3,Vesicle protein sorting 35,MEM3

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0770

规格

25μl100μl1ml

数量

产品介绍

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产品规格

宿主来源

Rabbit
抗原名称

VPS35
分子别名

Vacuolar protein sorting-associated protein 35, hVPS35, Maternal-embryonic 3, Vesicle protein sorting 35, MEM3
免疫原

Synthetic Peptide
细胞定位

Cytoplasm
Accession

Q96QK1
克隆号

S-997-2
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu, Ms, Rt
预测反应种属
(反应种属缩写表)

Bv
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

IHC-P ?

WB

IP
稀释度

应用稀释度推荐种属

WB 1:1000 Hu, Ms, Rt
IHC-P 1:250 Hu, Ms, Rt
IP 1:50 Hu

应用	稀释度	推荐种属
WB	1:1000	Hu, Ms, Rt
IHC-P	1:250	Hu, Ms, Rt
IP	1:50	Hu

背景介绍

Vacuolar protein sorting ortholog 35 (VPS35) is a protein involved in autophagy and is implicated in neurodegenerative diseases, such as Parkinson's disease (PD) and Alzheimer's disease (AD). VPS35 is part of a complex called the retromer, which is responsible for transporting select cargo proteins between vesicular structures (e.g., endosomes, lysosomes, vacuoles) and the Golgi apparatus. Mutations in the VPS35 cause aberrant autophagy, where cargo proteins fail to be transported and dysfunctional or unnecessary proteins fail to be degraded. There are numerous pathways affected by altered VPS35 levels and activity, which have clinical significance in neurodegeneration.

免疫印迹
- WB result of VPS35 Recombinant Rabbit mAb
  Primary antibody: VPS35 Recombinant mAb at 1/1000 dilution
  Lane 1: HepG2 whole cell lysate 20 µg
  Lane 2: A549 whole cell lysate 20 µg
  Lane 3: HEK-293 whole cell lysate 20 µg
  Lane 4: Raji whole cell lysate 20 µg
  Lane 5: Molt-4 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 91 kDa
  Observed MW: 91 kDa
- WB result of VPS35 Recombinant Rabbit mAb
  Primary antibody: VPS35 Recombinant mAb at 1/1000 dilution
  Lane 1: NIH/3T3 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 91 kDa
  Observed MW: 91 kDa
- WB result of VPS35 Recombinant Rabbit mAb
  Primary antibody: VPS35 Recombinant mAb at 1/1000 dilution
  Lane 1: C6 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 91 kDa
  Observed MW: 91 kDa
免疫沉淀
- VPS35 Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating VPS35 in 0.4 mg Raji whole cell lysate.
  Western blot was performed on the immunoprecipitate using VPS35 Rabbit mAb at 1/1000 dilution.
  Secondary antibody (HRP) for IP was used at 1/1000 dilution.
  Lane 1: Raji whole cell lysate 20 µg (Input)
  Lane 2: VPS35 Rabbit mAb IP in Raji whole cell lysate
  Lane 3: Rabbit monoclonal IgG IP in Raji whole cell lysate
  Predicted MW: 91 kDa
  Observed MW: 91 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-VPS35 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human kidney. Anti-VPS35 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-VPS35 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-VPS35 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human testis. Anti-VPS35 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse testis. Anti-VPS35 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat kidney. Anti-VPS35 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.