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宿主来源
Mouse抗原名称
CD52分子别名
CAMPATH-1 antigen, CDw52, Cambridge pathology 1 antigen, Epididymal secretory protein E5, Human epididymis-specific protein 5 (He5), HE5细胞定位
Cell membraneAccession
P31358克隆号
S-R445抗体类型
Mouse mAb抗体同种型
IgG2a,k反应种属 ?
Hu纯化方式
Protein A浓度
2 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
FCM
稀释度
应用 稀释度 FCM 1:2000
CD52 protein is a member of an unnamed short-chain GPI-anchored glycoprotein family in humans, which also includes human CD24 antigen and its homologues. CD52 protein is widely distributed and can be found on lymphocytes, monocytes, eosinophils, and dendritic cells derived from monocytes in the hematopoietic system, with a high density of up to 5×10^5 molecules per lymphocyte. In addition, CD52 antigen is also expressed to varying degrees on many lymphocytic malignancies and some acute myeloid leukemia cells. The CD52 protein may also play a role in the transport and targeting of carbohydrates, indicating that it participates in cell processes related to carbohydrate transport.
流式分析
Flow cytometric analysis of human PBMC (human peripheral blood mononuclear cell) labelling CD52 antibody at 1/2000 (0.1 μg) dilution (Right) compared with a Mouse monoclonal IgG isotype control (Left). Goat Anti - Mouse IgG Alexa Fluor® 488 was used as the secondary antibody. Then cells were stained with CD3 - Alexa Fluor® 647 separately. Gated on viable lymphocytes.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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