Phospho-Histone H3 (Ser10) Rabbit Polyclonal Antibody

H3S10P

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0751

规格

25μl100μl1ml

数量

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产品规格

宿主来源

Rabbit
抗原名称

Phospho-Histone H3 (Ser10)
分子别名

H3S10P
免疫原

Synthetic Peptide
细胞定位

Nucleus
Accession

P68431
抗体同种型

IgG
翻译后修饰类型

磷酸化
应用

ChIP ,
WB
稀释度

应用稀释度

Dot Blot 1:1000
WB 1:1000
ChIP 1:20-1:50
反应种属 ?

Hu
预测反应种属
(反应种属缩写表)

Fu, Pl, Ys, SeUr, Dr, C.el, Hm, Or, Bv, Xe
纯化方式

Immunogen Affinity
浓度

4 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied

应用	稀释度
Dot Blot	1:1000
WB	1:1000
ChIP	1:20-1:50

背景介绍

H3S10P is an epigenetic modification to the DNA packaging protein histone H3. It is a mark that indicates the phosphorylation the 10th serine residue of the histone H3 protein. The S10 phosphorylation is involved in mitosis, transcription, chromatin condensation, and UVB response. H3S10p causes chromosome condensation and segregation during cell mitosis. H3S10p temporarily increases during mitosis while H3K9me3 decareses and H3K9me3 recovers upon mitotic exit.

免疫印迹
- WB result of Phospho-Histone H3 (Ser10) Rabbit pAb
  Primary antibody: Phospho-Histone H3 (Ser10) Rabbit pAb at 1/1000 dilution
  Lane 1: untreated HeLa whole cell lysate 20 µg
  Lane 2: HeLa treated with 100 ng/ml Nocodazole for 18 hours, then treated with 100 nM Calyculin A for 1 hr whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 15 kDa
  Observed MW: 17 kDa
免疫沉淀
斑点杂交
- Dot blot result of Phospho-Histone H3 (Ser10) Rabbit pAb
  Lane1: Histone H3 (Ser10) phospho peptide
  Lane2: Histone H3 (Ser10) phospho peptide
  Lane3: Histone H3 non-phospho peptide
  Primary antibody: Phospho-Histone H3 (Ser10) Rabbit pAb at 1/1000 dilution
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
ChIP
- Chromatin immunoprecipitation (ChIP) was performed on HeLa + Nocodazole (100ng/ml,18h) (+) (upper panel) and HeLa + Nocodazole (100ng/ml,18h) (-) (lower panel) cells cross - linked with 1% formaldehyde for 10 min, then chromatin was fragmented bysonication.
  Parallel reactions used Phospho-Histone H3 (Ser10) Rabbit Polyclonal Antibody and Rabbit mAb IgG Isotype Control (SDT-R173) at 1:50 for immunoprecipitation.
  Post - immunoprecipitation, both samples were washed, eluted, and cross - links reversed. Purified DNA was analyzed by qPCR.
  qPCR (%input: immunoprecipitated DNA/input DNA)
  showed the enrichment of RPL30 and GAPDH in Phospho-Histone H3
  (Ser10) Rabbit Polyclonal Antibody -immunoprecipitated sample.