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宿主来源
Rabbit分子别名
Signal transducer and activator of transcription 1-alpha/beta; Transcription factor ISGF-3 components p91/p84免疫原
Synthetic Peptide细胞定位
NucleusAccession
P42224克隆号
S-601-78抗体类型
Recombinant mAb抗体同种型
IgG翻译后修饰类型
磷酸化应用
ChIP ,ICC ? ,WB反应种属 ?
Hu, Ms, Rt预测反应种属
(反应种属缩写表)Pg纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 |
|---|---|
| Dot Blot | 1:1000 |
| WB | 1:1000 |
| ICC | 1:100 |
| ChIP | 1:20-1:50 |
Stat1 (Signal Transducer and Activator of Transcription 1) is a crucial signaling molecule that plays a pivotal role in cells. Upon stimulation by external factors such as cytokines and growth factors, Stat1 is activated through tyrosine kinases (JAK) and mitogen-activated protein kinases (MAPK), leading to the phosphorylation of tyrosine and serine residues at its C-terminus. Phosphorylated Stat1 forms dimers and translocates into the nucleus, binding to specific DNA sequences to activate the transcription of target genes. Stat1 plays a crucial role in the immune system as a key component of the interferon signaling pathway. It regulates cell proliferation, apoptosis, and differentiation, affecting the growth and migration of tumor cells. Phospho-Stat1 (Tyr701) is a phosphorylated form of Stat1. It results from the phosphorylation of Stat1 at the Tyr701 residue. This phosphorylation event is typically induced by cytokines and growth factors through JAK (Janus kinase) or MAPK (Mitogen-Activated Protein Kinase) signaling pathways. In the nucleus, Phospho-Stat1 (Tyr701) dimers bind to specific DNA sequences known as gamma-activated sequence (GAS) elements in the promoters of target genes. Binding to these sequences leads to the transcriptional activation of those genes, thereby influencing cellular responses to cytokines and growth factors.
免疫印迹
WB result of Phospho-Stat1 (Tyr701) Recombinant Rabbit mAb
Primary antibody: Phospho-Stat1 (Tyr701) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated HeLa whole cell lysate 20 µg
Lane 2: HeLa treated with 100 ng/ml IFN-γ for 30 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 87 kDa
Observed MW: 91 kDaWB result of Phospho-Stat1 (Tyr701) Recombinant Rabbit mAb
Primary antibody: Phospho-Stat1 (Tyr701) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated A20 whole cell lysate 20 µg
Lane 2: A20 treated with 10 ng/ml hIFN-α for 30 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 87 kDa
Observed MW: 84, 91 kDaWB result of Phospho-Stat1 (Tyr701) Recombinant Rabbit mAb
Primary antibody: Phospho-Stat1 (Tyr701) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated PC-12 whole cell lysate 20 µg
Lane 2: PC-12 treated with 10 ng/ml hIFN-α for 30 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 87 kDa
Observed MW: 91 kDa
斑点杂交
Dot blot result of Phospho-Stat1 (Tyr701) Recombinant Rabbit mAb
Lane1: Stat1 (Tyr701) phospho peptide
Lane2: Stat1 non-phospho peptide
Primary antibody: Phospho-Stat1 (Tyr701) Recombinant Rabbit mAb at 1/1000 dilution
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
免疫细胞化学
ICC analysis of HeLa cells treated with IFN-γ (100ng/mL, 30min) (top panel) and HeLa cells untreated with IFN-γ (100ng/mL, 30min) (below panel). Anti- Phospho-Stat1 (Tyr701) antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ChIP
Chromatin immunoprecipitation (ChIP) was performed on HeLa + IFN-α (50 ng/ml, 30 min) cells cross - linked with 1% formaldehyde for 10 min, then chromatin was fragmented by sonication. Parallel reactions used Phospho-Stat1 (Tyr701) Recombinant Rabbit mAb (S-601-78) and Rabbit mAb IgG Isotype Control (SDT-R173) at 1:50 for immunoprecipitation.
Post - immunoprecipitation, both samples were washed, eluted, and cross - links reversed. Purified DNA was analyzed by qPCR.
qPCR showed the enrichment of IRF1, TAP1 and SAT-α in Phospho-Stat1 (Tyr701)
Recombinant Rabbit mAb (S-601-78)-immunoprecipitated sample.
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斯达特公司的抗体，可以回收利用几次？
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检测磷酸化蛋白时，推荐有什么封闭体系？
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