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Rabbit分子别名
Induced myeloid leukemia cell differentiation protein Mcl-1, Bcl-2-like protein 3 (Bcl2-L-3), Bcl-2-related protein EAT/mcl1, mcl1/EAT, BCL2L3免疫原
Synthetic Peptide细胞定位
Cytoplasm, NucleusAccession
Q07820克隆号
S-772-109抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Rt预测反应种属
(反应种属缩写表)Ct, Dg纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu,Rt IHC-P 1:250 Hu,Rt ICC 1:500 Hu
MCL1 (Myeloid Cell Leukemia 1) is a protein that belongs to the Bcl-2 (B-cell lymphoma 2) family of proteins. This family of proteins plays a vital role in regulating cell death, specifically apoptosis, a process that removes unwanted or damaged cells from the body. MCL1 expression is regulated by various signaling pathways and transcription factors. Its levels can be increased in response to growth factors and cytokines, promoting cell survival. Conversely, MCL1 levels can be decreased during stress conditions or by specific targeted therapies, leading to apoptosis. MCL1 is frequently overexpressed in various types of cancers, including leukemia, lymphoma, and solid tumors. Its anti-apoptotic function allows cancer cells to survive and proliferate despite damage or stress. As a result, MCL1 has been identified as a potential target for cancer therapy. Inhibiting MCL1 activity or reducing its expression can promote apoptosis in cancer cells, leading to tumor regression.
免疫印迹
WB result of MCL1 Recombinant Rabbit mAb
Primary antibody: MCL1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: MCF-7 whole cell lysate 20 µg
Lane 2: Raji whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 37 kDa
Observed MW: 34, 37 kDaWB result of MCL1 Recombinant Rabbit mAb
Primary antibody: MCL1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat colon lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 37 kDa
Observed MW: 30, 35 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human tonsil. Anti-MCL1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-MCL1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-MCL1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-MCL1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat colon. Anti-MCL1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows high expression in MCF-7 cells (top panel) and low expression in SK-OV-3 cells (below panel). Anti-MCL1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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