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Rabbit抗原名称
GM-CSF分子别名
Granulocyte-macrophage colony-stimulating factor, Colony-stimulating factor (CSF), Molgramostin, Sargramostim, CSF2, GMCSF免疫原
Recombinant Protein细胞定位
SecretedAccession
P04141克隆号
S-725-327抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
WB
IP
稀释度
应用 稀释度 WB 1:5000 IP 1:50
GM-CSF is an acidic glycoprotein with a molecular weight of approximately 23kDa, containing internal disulfide bonds. It is primarily produced by mesenchymal cells present in the hematopoietic environment and peripheral inflammatory sites, responding to various inflammatory mediators. GM-CSF stimulates the formation of granulocytes and macrophages from bone marrow precursor cells, promoting the production of neutrophil, macrophage, and mixed granulocyte-macrophage colonies. It can also stimulate eosinophil colony formation from fetal liver progenitor cells. GM-CSF is generally undetectable in the blood of healthy individuals, but its levels may increase under certain conditions such as inflammation or cancer treatment. It stimulates functional activities in mature granulocytes and macrophages. GM-CSF plays a crucial role in maintaining lung homeostasis as a key homeostatic factor in the alveoli. At low levels, it promotes the development and long-term maintenance of alveolar macrophages. In the absence of GM-CSF, there is a defect in alveolar macrophage function, increasing the risk of pulmonary infections and leading to lung diseases. Abnormal expression of GM-CSF can also lead to symptoms such as inflammation, pain, tissue damage, and may promote the production of other pathogenic cytokines.
免疫印迹
WB result of GM-CSF Recombinant Rabbit mAb
Primary antibody: GM-CSF Recombinant Rabbit mAb at 1/5000 dilution
Lane 1: untreated NK-92 whole cell lysate 20 µg
Lane 2: NK-92 treated with 80 nM TPA and 3 μM Ionomycin for 6 hours, then with 300 ng/mL Brefeldin A added for 4 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 16 kDa
Observed MW: 14~25 kDa
免疫沉淀
GM-CSF Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating GM-CSF in 0.4 mg NK-92 treated with 80 nM TPA and 3 μM Ionomycin for 6 hours, then with 300 ng/mL Brefeldin A added for 4 hours whole cell lysate.
Western blot was performed on the immunoprecipitate using GM-CSF Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/1000 dilution.
Lane 1:NK-92 treated with 80 nM TPA and 3 μM Ionomycin for 6 hours, then with 300 ng/mL Brefeldin A added for 4 hours whole cell lysate 20 µg (Input)
Lane 2: GM-CSF Rabbit mAb IP in NK-92 treated with 80 nM TPA and 3 μM Ionomycin for 6 hours, then with 300 ng/mL Brefeldin A added for 4 hours whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in NK-92 treated with 80 nM TPA and 3 μM Ionomycin for 6 hours, then with 300 ng/mL Brefeldin A added for 4 hours whole cell lysate
Predicted MW: 16 kDa
Observed MW: 14~25 kDa
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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