Beclin-1 Recombinant Rabbit mAb (S-761-15)

Coiled-coil myosin-like BCL2-interacting protein,Protein GT197,BECN1,GT197,Beclin 1

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0739

规格

25μl100μl1ml

数量

产品介绍

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产品规格

宿主来源

Rabbit
分子别名

Coiled-coil myosin-like BCL2-interacting protein, Protein GT197, BECN1, GT197,Beclin 1
免疫原

Recombinant Protein
细胞定位

Cytoplasm
Accession

Q14457
克隆号

S-761-15
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu, Ms, Rt
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

IHC-P ? ,
WB
稀释度

应用稀释度推荐种属

WB 1:1000 Hu, Ms, Rt
IHC-P 1:100 Hu, Ms, Rt

应用	稀释度	推荐种属
WB	1:1000	Hu, Ms, Rt
IHC-P	1:100	Hu, Ms, Rt

背景介绍

Beclin-1 is a mammalian ortholog of the yeast autophagy-related gene 6 (Atg6) and BEC-1 in the C. elegans nematode. This protein interacts with either BCL-2 or PI3k class III, playing a critical role in the regulation of both autophagy and cell death. Beclin-1 plays an important role in tumorigenesis, and neurodegeneration, being implicated in the autophagic programmed cell death. Ovarian cancer with upregulated autophagy has a less aggressive behavior and is more responsive to chemotherapy. Schizophrenia is associated with low levels of Beclin-1 in the hippocampus of those affected, which causes diminished autophagy which in turn results in increased neuronal cell death.

免疫印迹
- WB result of Beclin-1 Recombinant Rabbit mAb
  Primary antibody: Beclin-1 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: HeLa whole cell lysate 20 µg
  Lane 2: HepG2 whole cell lysate 20 µg
  Lane 3: MCF7 whole cell lysate 20 µg
  Lane 4: Jurkat whole cell lysate 20 µg
  Lane 5: Raji whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 52 kDa
  Observed MW: 55 kDa
- WB result of Beclin-1 Recombinant Rabbit mAb
  Primary antibody: Beclin-1 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: RAW 264.7 whole cell lysate 20 µg
  Lane 2: NIH/3T3 whole cell lysate 20 µg
  Lane 3: C2C12 whole cell lysate 20 µg
  Lane 4: mouse kidney lysate 20 µg
  Lane 5: mouse spleen lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 52 kDa
  Observed MW: 55 kDa
- WB result of Beclin-1 Recombinant Rabbit mAb
  Primary antibody: Beclin-1 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: PC-12 whole cell lysate 20 µg
  Lane 2: rat kidney lysate 20 µg
  Lane 5: rat spleen lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 52 kDa
  Observed MW: 55 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human testis. Anti- Beclin-1 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human breast cancer. Anti- Beclin-1 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti- Beclin-1 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse testis. Anti- Beclin-1 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat kidney. Anti- Beclin-1 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.