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宿主来源
Rabbit分子别名
Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau (PHF-tau), MAPT, MAPTL, MTBT1免疫原
Synthetic Peptide细胞定位
CytoplasmAccession
P10636-8克隆号
S-982-20抗体类型
Recombinant mAb抗体同种型
IgG翻译后修饰类型
磷酸化反应种属 ?
Ms预测反应种属
(反应种属缩写表)Cz, Gor, Pr, Or, Hu, Rt, Mq, Bv, Sq, Gt, Bb纯化方式
Protein A浓度
2 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
WB稀释度
应用 稀释度 Dot Blot 1:1000 WB 1:1000
Tau protein is a microtubule-associated protein (MAP) that is abundantly expressed in neurons of the central nervous system and is scarcely found in other cell types. It primarily contributes to maintaining the structure and stability of microtubules, which are crucial for the normal function and axonal transport of neurons. Phosphorylation is an important mode of regulating Tau protein function. Abnormal levels or functions of Tau (phospho S404) may be associated with specific types of neurodegenerative diseases.
免疫印迹
WB result of Tau (phospho S404) Recombinant Rabbit mAb
Primary antibody: Tau (phospho S404) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: old mouse brain lysate 20 µg
Lane 2: phosphatase treated old mouse brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 46 kDa
Observed MW: 48 kDa
斑点杂交
Dot blot result of Tau (phospho S404) Recombinant Rabbit mAb
Lane 1: Tau (phospho S404) peptide
Lane 2: Tau non-phospho peptide (around S404)
Primary antibody: Tau (phospho S404) Recombinant Rabbit mAb at 1/1000 dilution
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
This blot was developed with high sensitivity substrate
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
我们推荐客户使用TPST+5%脱脂奶粉来稀释一抗，进行封闭。 虽然BSA被推荐为WB检测磷酸化蛋白的常用封闭剂，但是脱脂奶粉获取更加方便，覆盖更广泛的非特异性结合位点，在一抗性能优越的前提下，使用脱脂奶粉封闭性价比更高
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