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Rabbit抗原名称
β-Actin分子别名
ACTB细胞定位
Cytoplasm, CytoskeletonAccession
P60709克隆号
S-R015抗体类型
Recombinant mAb抗体同种型
IgG应用
WB反应种属 ?
Hu, Ms, Rt纯化方式
Protein A浓度
1 mg/ml标记
HRP性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 |
|---|---|
| WB | 1:5000-1:10000 |
Beta-actin (human gene and protein abbreviation ACTB/ACTB) is one of six different actin isoforms which have been identified in humans. This is one of the two nonmuscle cytoskeletal actins. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Beta actin is often used in Western blotting as a loading control, to normalize total protein amounts and check for eventual protein degradation in the samples. Its transcript is also commonly used as a housekeeping gene standard in qPCR. Its molecular weight is approximately 42 kDa.
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WB result of β-actin Recombinant Rabbit mAb (HRP Conjugate)
Primary antibody: β-actin Recombinant Rabbit mAb (HRP Conjugate) at 1/10000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HepG2 whole cell lysate 20 µg
Predicted MW: 42 kDa
Observed MW: 42 kDaWB result of β-actin Recombinant Rabbit mAb (HRP Conjugate)
Primary antibody: β-actin Recombinant Rabbit mAb (HRP Conjugate) at 1/10000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Predicted MW: 42 kDa
Observed MW: 42 kDaWB result of β-actin Recombinant Rabbit mAb (HRP Conjugate)
Primary antibody: β-actin Recombinant Rabbit mAb (HRP Conjugate) at 1/10000 dilution
Lane 1: C6 whole cell lysate 20 µg
Predicted MW: 42 kDa
Observed MW: 42 kDa
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