KIF3A Recombinant Rabbit mAb (S-1083-9)

Kinesin-like protein KIF3A,Microtubule plus end-directed kinesin motor 3A

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0730

规格

25μl100μl1ml

数量

产品介绍

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产品规格

宿主来源

Rabbit
抗原名称

KIF3A
分子别名

Kinesin-like protein KIF3A, Microtubule plus end-directed kinesin motor 3A
免疫原

Synthetic Peptide
细胞定位

Cytoplasm, Cytoskeleton
Accession

Q9Y496
克隆号

S-1083-9
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu, Ms, Rt
预测反应种属
(反应种属缩写表)

Mq, Or
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

ICFCM

IHC-P ?

ICC ?

WB

IP
稀释度

应用稀释度推荐种属

WB 1:1000 Hu, Ms, Rt
IP 1:50 Hu
IHC-P 1:250 Hu, Ms, Rt
ICC 1:50 Hu
ICFCM 1:500 Hu

应用	稀释度	推荐种属
WB	1:1000	Hu, Ms, Rt
IP	1:50	Hu
IHC-P	1:250	Hu, Ms, Rt
ICC	1:50	Hu
ICFCM	1:500	Hu

背景介绍

KIF3A is a subunit of the heterotrimeric kinesin-2 complex, which belongs to the N-kinesin family of microtubule plus-end-directed motor proteins. Along with KIF3B and the cargo-binding protein KAP3, it forms the heterotrimeric kinesin-2 complex that is involved in regulating intracellular transport and mediating microtubule-based motility. The specific functions of KIF3A include roles in regulating bone formation, nephron number, cell survival, and gene expression control. It is also implicated in sperm formation, prostate cancer suppression, and glioblastoma, among others. KIF3A is associated with diseases such as Usher syndrome type I and neuronal ceroid lipofuscinosis.

免疫印迹
- WB result of KIF3A Recombinant Rabbit mAb
  Primary antibody: KIF3A Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: HeLa whole cell lysate 20 µg
  Lane 2: SH-SY5Y whole cell lysate 20 µg
  Lane 3: 293T whole cell lysate 20 µg
  Lane 4: MDA-MB-231 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 80 kDa
  Observed MW: 80 kDa
  This blot was developed with high sensitivity substrate
- WB result of KIF3A Recombinant Rabbit mAb
  Primary antibody: KIF3A Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: mouse brain lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 80 kDa
  Observed MW: 80 kDa
  This blot was developed with high sensitivity substrate
- WB result of KIF3A Recombinant Rabbit mAb
  Primary antibody: KIF3A Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: rat brain lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 80 kDa
  Observed MW: 80 kDa
  This blot was developed with high sensitivity substrate
流式分析
- Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) labelling KIF3A antibody at 1/500 dilution (0.1 μg)/ (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫沉淀
- KIF3A Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating KIF3A in 0.4 mg SH-SY5Y whole cell lysate.
  Western blot was performed on the immunoprecipitate using KIF3A Rabbit mAb at 1/1000 dilution.
  Secondary antibody (HRP) for IP was used at 1/1000 dilution.
  Lane 1: SH-SY5Y whole cell lysate 20 µg (Input)
  Lane 2: KIF3A Rabbit mAb IP in SH-SY5Y whole cell lysate
  Lane 3: Rabbit monoclonal IgG IP in SH-SY5Y whole cell lysate
  Predicted MW: 80 kDa
  Observed MW: 80 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human testis. Anti-KIF3A antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human cerebellum. Anti-KIF3A antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-KIF3A antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse cerebellum. Anti-KIF3A antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-KIF3A antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat cerebellum. Anti-KIF3A antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
- ICC shows positive staining in SH-SY5Y cells. Anti-KIF3A antibody was used at 1/50 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).