BiP Rabbit Polyclonal Antibody
Endoplasmic reticulum chaperone BiP,78 kDa glucose-regulated protein (GRP-78),Binding-immunoglobulin protein,Heat shock protein 70 family protein 5 (HSP70 family protein 5),Heat shock protein family A member 5,Immunoglobulin heavy chain-binding protein,HSPA5,GRP78
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宿主来源
Rabbit分子别名
Endoplasmic reticulum chaperone BiP; 78 kDa glucose-regulated protein (GRP-78); Binding-immunoglobulin protein; Heat shock protein 70 family protein 5 (HSP70 family protein 5); Heat shock protein family A member 5; Immunoglobulin heavy chain-binding protein; HSPA5; GRP78免疫原
Synthetic Peptide细胞定位
CytoplasmAccession
P11021抗体类型
Polyclonal antibody抗体同种型
IgG反应种属 ?
Hu, Ms, Rt预测反应种属
(反应种属缩写表)Xe, Bv, Hm, Sq, Or纯化方式
Immunogen Affinity浓度
1 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt IHC-P 1:200 Hu, Ms, Rt
The BiP protein, also known as Binding immunoglobulin protein or HSPA5, is a crucial molecular chaperone located in the endoplasmic reticulum (ER) that belongs to the Hsp70 family. BiP binds to hydrophobic amino acid residues of unfolded proteins entering the ER, preventing incorrect folding and aggregation. It also interacts with folded proteins to protect them from aggregation and degradation. Under ER stress conditions, BiP interacts with ER stress sensors such as ATF6, IRE1α, and PERK, regulating their activation and initiating the ER stress response pathway to help cells cope with unfolded protein accumulation. Due to its essential role in the cell, abnormal expression or dysfunction of BiP is closely related to the development of various diseases. For instance, BiP is overexpressed in tumor cells, associated with tumor invasion, metastasis, and drug resistance. Additionally, some genetic and neurodegenerative diseases are linked to abnormal expression or dysfunction of BiP.
免疫印迹
WB result of BiP Rabbit pAb
Primary antibody: BiP Rabbit pAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: MCF7 whole cell lysate 20 µg
Lane 3: HepG2 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 72 kDa
Observed MW: 78 kDaWB result of BiP Rabbit pAb
Primary antibody: BiP Rabbit pAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: mouse liver lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 72 kDa
Observed MW: 78 kDaWB result of BiP Rabbit pAb
Primary antibody: BiP Rabbit pAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Lane 2: rat liver lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 72 kDa
Observed MW: 78 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human liver. Anti-BiP antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-BiP antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-BiP antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-BiP antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of BiP in tumor tissue.
Expression of BiP in human tissue.
Expression of BiP in mouse & rat tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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