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Rabbit抗原名称
RPE65分子别名
Retinoid isomerohydrolase, All-trans-retinyl-palmitate hydrolase, Lutein isomerase, Meso-zeaxanthin isomerase, Retinal pigment epithelium-specific 65 kDa protein, Retinol isomerase免疫原
Synthetic Peptide细胞定位
Cell membrane, CytoplasmAccession
Q16518克隆号
S-525-124抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt预测反应种属
(反应种属缩写表)Dg, Pr纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
IP
IF ?
稀释度
应用 稀释度 推荐种属 WB 1:1000 Ms, Rt IP 1:50 Ms IHC-P 1:1000 Ms, Rt IF 1:1000 Ms, Rt
The RPE65 protein, also known as Retinoid isomerohydrolase, is a crucial protein produced in the retinal pigment epithelium (RPE) cells. PE65 protein serves as a key isomerohydrolase in the visual cycle, involved in the regeneration of 11-cis-retinal, which is the chromophore of rod and cone cell visual pigments. It catalyzes the cleavage and isomerization of all-trans-retinyl fatty acid esters to produce 11-cis-retinol, which is further oxidized by 11-cis-retinol dehydrogenase to 11-cis-retinal. RPE65 protein exists in two forms: soluble sRPE65 and membrane-bound mRPE65. The latter is palmitoylated and serves as a palmitoyl donor for lecithin retinol acyltransferase (LRAT), which catalyzes a step in the regeneration of vitamin A to all-trans-retinol.
免疫印迹
WB result of RPE65 Recombinant Rabbit mAb
Primary antibody: RPE65 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse eye lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 61 kDa
Observed MW: 65 kDaWB result of RPE65 Recombinant Rabbit mAb
Primary antibody: RPE65 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat eye lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 61 kDa
Observed MW: 65 kDa
免疫沉淀
RPE65 Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating RPE65 in 0.4 mg mouse eye lysate.
Western blot was performed on the immunoprecipitate using RPE65 Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/1000 dilution.
Lane 1: mouse eye lysate 20 µg (Input)
Lane 2: RPE65 Rabbit mAb IP in mouse eye lysate
Lane 3: Rabbit monoclonal IgG IP in mouse eye lysate
Predicted MW: 61 kDa
Observed MW: 65 kDa
免疫组化
IHC shows positive staining in paraffin-embedded mouse retina. Anti-RPE65 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat retina. Anti-RPE65 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫荧光
IF shows positive staining in paraffin-embedded mouse retina. Anti-RPE65 antibody was used at 1/1000 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded rat retina. Anti-RPE65 antibody was used at 1/1000 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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