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宿主来源
Mouse分子别名
mCherry fluorescent protein免疫原
Recombinant ProteinAccession
X5DSL3克隆号
S-1050-13抗体类型
Mouse mAb抗体同种型
IgG2b,k反应种属 ?
Species Independent交叉反应
This antibody is reactive against variants of RFP such asmRFP, mPlum, mOrange, PA-mCherry and mScarlet
纯化方式
Protein A浓度
2 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
ICFCM
ChIP
WB
稀释度
应用 稀释度 WB 1:5000-1:100000 ICFCM 1:2000 ChIP 1:20-1:50
mCherry is a widely used red fluorescent protein in biotechnology, exhibiting several key properties and applications. mCherry originates from the coral Discosoma species and serves as a monomeric red fluorescent protein. It absorbs light in the range of 540-590 nm and emits light in the range of 550-650 nm. Unlike GFP (Green Fluorescent Protein) and its variants derived from jellyfish, mCherry possesses distinct red fluorescent properties. It is commonly used as a red fluorescent tag protein for labeling and localizing molecular components within cells.
免疫印迹
WB result of mCherry Mouse mAb
Primary antibody: mCherry Mouse mAb at 1/5000 dilution
Lane 1: untreated 293T whole cell lysate 20 µg
Lane 2: 293T transfected with Histone H3-mCherry-E-tag whole cell Lysate 20 µg
Secondary antibody: Goat Anti-mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 27 kDa
Observed MW: 48 kDa
流式分析
Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized Histone H3-mCherry-E-Tag transfected 293T (Human embryonic kidney epithelial cell) labelling CBP Tag antibody at 1/2000 (0.1 μg) dilution/ (Right panel) compared with a Mouse IgG Isotype Control / (Left panel). Goat Anti - Mouse IgG Alexa Fluor® 488 was used as the secondary antibody.
ChIP
Chromatin immunoprecipitation (ChIP) was performed on 293F cells were either untransfected (left panel) or transfected with an mCherry-tagged human H3 construct (right panel) cross - linked with 1% formaldehyde for 10 min, then chromatin was fragmented by sonication. Parallel reactions used mCherry Mouse mAb (S-1050-13) and Mouse mAb IgG1, κ Isotype Control (S-844-79) at 1:50 for immunoprecipitation.
Post-immunoprecipitation, both samples were washed, eluted, and cross-links reversed. Purified DNA was analyzed by qPCR.
qPCR showed the enrichment of RPL30, AFM and SAT-α
in mCherry Mouse mAb (S-1050-13)-
immunoprecipitated sample.
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