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Rabbit分子别名
mCherry fluorescent protein免疫原
Recombinant ProteinAccession
X5DSL3克隆号
S-1049-67抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Species Independent交叉反应
This antibody is reactive against variants of RFP such asmRFP, mPlum, mOrange, PA-mCherry and mScarlet
纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
ChIP
WB
稀释度
应用 稀释度 WB 1:1000-1:5000 ChIP 1:20-1:50
mCherry is a widely used red fluorescent protein in biotechnology, exhibiting several key properties and applications. mCherry originates from the coral Discosoma species and serves as a monomeric red fluorescent protein. It absorbs light in the range of 540-590 nm and emits light in the range of 550-650 nm. Unlike GFP (Green Fluorescent Protein) and its variants derived from jellyfish, mCherry possesses distinct red fluorescent properties. It is commonly used as a red fluorescent tag protein for labeling and localizing molecular components within cells.
免疫印迹
WB result of mCherry Recombinant Rabbit mAb
Primary antibody: mCherry Recombinant Rabbit mAb at 1/5000 dilution
Lane 1: 293T whole cell lysate 20 µg
Lane 2: 293T transfected with empty vector whole cell Lysate 20 µg
Lane 3: 293T transfected with Histone H3-mCherry-E-tag whole cell Lysate 5 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 27 kDa
Observed MW: 48 kDa
ChIP
Chromatin immunoprecipitation (ChIP) was performed on 293F cells were either untransfected (left panel) or transfected with a mCherry-tagged human H3 construct (right panel) cross - linked with 1% formaldehyde for 10 min, then chromatin was fragmented by sonication. Parallel reactions used mCherry Recombinant Rabbit mAb (S-1049-67) and Rabbit mAb IgG Isotype Control (SDT-R173) at 1:50 for immunoprecipitation.
Post-immunoprecipitation, both samples were washed, eluted, and cross-links reversed. Purified DNA was analyzed by qPCR.
qPCR showed the enrichment of RPL30, AFM and SAT-α
in mCherry Recombinant Rabbit mAb (S-1049-67)-
immunoprecipitated sample.
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