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宿主来源
Rabbit分子别名
C-X-C chemokine receptor type 3, CXC-R3, CXCR-3, CKR-L2, G protein-coupled receptor 9, Interferon-inducible protein 10 receptor (IP-10 receptor), CD183, GPR9免疫原
Synthetic Peptide细胞定位
Cell membraneAccession
P49682克隆号
S-377-8抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
稀释度
应用 稀释度 IHC-P 1:2000
CXCR3, also known as C-X-C chemokine receptor type 3, is a G-protein-coupled receptor primarily expressed on the surface of activated T cells, B cells, and natural killer (NK) cells. It functions by binding to specific ligands on target cell membranes, inducing targeted migration and immune responses. CXCR3 plays a significant role in infection, autoimmune diseases, and tumor immunology. As a receptor for CXC chemokines such as CXCL9, CXCL10, and CXCL11, CXCR3 affects human mesangial cells (HMC) and other cell types through G-protein signaling. CXCR3 may also bind to CCL21, potentially contributing to chemotactic responses. Additionally, CXCR3 plays a crucial role in directing the migration of effector T cells to sites of inflammation and tumors, with its activation preferentially leading to high expression in Th1-type CD4+ T lymphocytes, effector CD8+ T lymphocytes, NK cells, and NKT cells. In recent years, numerous studies have confirmed that the expression of CXCR3 and its ligands in the body is closely related to tumor immunity, tumorigenesis, and metastasis. Therefore, CXCR3 has the potential to become a new indicator for assessing the prognosis of tumor patients and a new target for clinical tumor immunotherapy.
免疫组化
IHC shows positive staining in paraffin-embedded human tonsil. Anti-CXCR3 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human stomach. Anti-CXCR3 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human Hodgkin’s lymphoma. Anti-CXCR3 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-CXCR3 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-CXCR3 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human pancreatic carcinoma. Anti-CXCR3 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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